Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency

Patent metadata

Field	Value
Publication number	US-10000739-B2
Application number	US-201113881678-A
Country	US
Kind code	B2
Filing date	Oct 20, 2011
Priority date	Oct 28, 2010
Publication date	Jun 19, 2018
Grant date	Jun 19, 2018

Abstract

The purpose of the present invention is to provide a method and a culture medium which are capable of efficiently inducing the differentiation of pluripotent stem cells such as ES cells and iPS cells into desired cells by a simple means. The differentiation of pluripotent stem cells can be induced by culturing mammal-derived pluripotent stem cells in a differentiation culture medium that does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine. Also provided is a differentiation-inducing culture medium which does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine.

First claim

The invention claimed is:

1. A method of inducing differentiation of pluripotent stem cells into cells expressing endoderm, mesoderm or ectoderm markers, comprising the following steps:
(a) obtaining human pluripotent stem cells, wherein the human pluripotent stem cells are human embryonic stem cell (hESCs) or human induced pluripotent stem cells (hiPSCs),
(b) culturing the human pluripotent stem cells of step (a) in a culture medium comprising threonine, methionine, valine, leucine, isoleucine, phenylalanine, tryptophan, lysine, histidine, cysteine, tyrosine and arginine, to produce cells expressing endoderm, mesoderm or ectoderm markers, and
(c) culturing the cells of step (b) in a methionine-deprived culture medium comprising threonine, valine, isoleucine, phenylalanine, tryptophan, lysine and histidine for at least 5 hours and not more than 4 days, to eliminate Oct3/4 undifferentiated pluripotent stem cells, thereby producing an enriched population of induced differentiated cells expressing endoderm, mesoderm or ectoderm markers.

2. The method of claim 1 , wherein the cells of step (c) are further cultured in an endoderm differentiation culture medium comprising Activin A and B27 to produce endoderm cells expressing Sox17.

3. The method according to claim 2 , wherein the differentiated endoderm cells are further cultured in hepatocyte differentiation culture medium, comprising dexamethasone, human recombinant hepatocyte growth factor (HGF), nicotinamide and ascorbic acid, to produce hepatocytes expressing alpha-fetoprotein (AFP).

4. The method according to claim 2 , wherein the differentiated endoderm cells are further cultured in a pancreas differentiation culture medium, comprising glucose, retinoic acid, FGF10, KAAF-cyclomine and B27, to produce pancreatic cells expressing PDX1.

5. The method according to claim 3 , wherein the hepatocyte differentiation culture medium contains proline added in an amount of 1 mM or more and 10 mM or less.

6. The method according to claim 4 , wherein the pancreas differentiation culture medium contains proline added in an amount of 1 mM or more and 10 mM or less.

Assignees

• Kume Shoen
• Endo Fumio
• Shiraki Nobuaki
• Shiraki Yasuko
• Kume Kazuhiko
• Univ Kumamoto Nat Univ Corp

Inventors

• Kume Shoen
• Endo Fumio
• Shiraki Nobuaki
• Shiraki Yasuko
• Kume Kazuhiko

Classifications

• C12N2506/45

  from artificially induced pluripotent stem cells · CPC title

• C12N2506/02

  from embryonic cells · CPC title

• C12N2500/32

  Amino acids · CPC title

• C12N5/067 Primary

  Hepatocytes · CPC title

• C12N2533/52

  Fibronectin; Laminin · CPC title