Method to introduce Salmonella into ground meat

Patent metadata

Field	Value
Publication number	US-9995735-B2
Application number	US-201615261335-A
Country	US
Kind code	B2
Filing date	Sep 9, 2016
Priority date	Sep 10, 2015
Publication date	Jun 12, 2018
Grant date	Jun 12, 2018

Abstract

The present invention includes a method of introducing a pathogenic infection into one or more peripheral lymph nodes of an animal for testing of meat, comprising: inoculating at one or more peripheral lymph node drainage areas the animal with a known amount of a known pathogen; harvesting one or more peripheral lymph nodes from the animal; grinding meat or meat trimmings and the one or more peripheral lymph nodes into ground meat; and determining a ratio of a number of peripheral lymph nodes infected to the weight of the meat or meat trimmings used to create the ground meat, wherein the infected ground meat can be used to test interventions against the known pathogen in a grinding process.

First claim

What is claimed is:

1. A method of introducing a pathogenic infection into one or more peripheral lymph nodes of an animal at one or more peripheral lymph node drainage areas for testing of meat, comprising:
inoculating at one or more peripheral lymph node drainage areas of the animal with a known amount of one or more known pathogens, wherein each of the one or more peripheral lymph node drainage areas comprise multiple lymph nodes;
harvesting the peripheral lymph nodes of the peripheral lymph node drainage areas at the site of inoculation from the animal;
separating the peripheral lymph nodes into individual lymph nodes;
determining if the individual lymph node is infected with the one or more known pathogens;
grinding meat or meat trimmings at the site of inoculation into ground meat; and
determining a ratio of the number of individual lymph nodes infected to a total number of lymph nodes and a weight of the meat or meat trimmings used to create the ground meat, wherein the infected ground meat can be used to test interventions against the known pathogen in a grinding process.

2. The method of claim 1 , wherein the inoculation is selected from at least one of an intradermal, subdermal or transdermal inoculation.

3. The method of claim 1 , wherein the pathogen is selected from at least one of Salmonella, Listeria, Yersinia, Campylobacter, Shigella, E. coli, Francisella, Clostridum, Staphylococcus, Streptococcus , or Bacillus , and strains thereof.

4. The method of claim 1 , wherein the lymph node drainage areas comprise at least one of subiliac, popliteal, retropharangeal, superficial cervical, and axillary.

5. The method of claim 1 , wherein the one or more known pathogens are selected from at least one of viral and protozoan pathogens.

6. The method of claim 1 , wherein the animals comprise bovine, equine, ovine, porcine, or caprine.

7. The method of claim 1 , further comprising the step of challenging the animal infected with the one or more pathogens at one or more sites with a therapeutic intervention to reduce or eliminate the one or more pathogens.

8. The method of claim 1 , further comprising the step of titrating the amount of the known pathogen used during the inoculating step to obtain a pre-determined distribution of infected peripheral lymph nodes.

9. The method of claim 1 , wherein the peripheral lymph nodes do not include gut associated lymph nodes.

10. The method of claim 1 , wherein the meat prior to grinding is sterile.

11. A method of introducing an indicator bacteria into one or more peripheral lymph nodes of an animal to determine if a therapy, treatment or exposure eliminates or reduces the indicator bacteria comprising:
inoculating at one or more sites of the animal a known amount of the indicator bacteria, wherein the one or more inoculation sites comprise one or more peripheral lymph node drainage areas;
treating the animal with one or more therapies, treatments, or exposure at one or more time points;
harvesting the one or more peripheral lymph nodes from the animal that comprise the inoculated peripheral lymph nodes;
grinding the one or more peripheral lymph nodes with meat or meat trimmings known to be sterile into ground meat; and
determining if the one or more therapies, treatments, or exposure were effective to eliminate or reduce the indicator bacteria in the ground meat.

12. The method of claim 11 , wherein the inoculation is selected from at least one of an intradermal, subdermal or transdermal inoculation.

13. The method of claim 11 , wherein the indicator bacteria is selected from Salmonella, Listeria, Yersinia, Campylobacter, Shigella, E. coli, Francisella, Clostridum, Staphylococcus, Streptococcus , or Bacillus.

14. The method of claim 11 , wherein the lymph node drainage areas comprise at least one of subiliac, popliteal, retropharangeal, superficial cervical, and axillary.

15. The method of claim 11 , wherein the animals comprise bovine, equine, ovine, porcine, or caprine.

16. The method of claim 11 , further comprising the step of titrating the amount of the indicator bacteria used during the inoculating step to obtain a pre-determined distribution of infected peripheral lymph nodes.

17. The method of claim 11 , wherein the peripheral lymph nodes do not include gut associated lymph nodes.

18. A method of testing a compound for elimination of bacterial infections within the lymph nodes of an animal comprising:
inoculating at one or more sites on the animal with a known amount of said bacteria, wherein the one or more inoculation sites comprise peripheral lymph node drainage areas;
treating the animal with one or more compounds at one or more time points;
harvesting from the animal the inoculated peripheral lymph nodes;
grinding the harvested peripheral lymph nodes with meat or meat trimmings that are sterile into ground meat; and
determining if the one or more compounds were effective to eliminate or reduce the bacteria.

19. The method of claim 18 , wherein the inoculation is selected from at least one of an subdermal or transdermal inoculation.

20. The method of claim 18 , wherein the bacteria is selected from Salmonella, Listeria, Yersinia, Campylobacter, Shigella, E. coli, Francisella, Clostridum, Staphylococcus, Streptococcus , or Bacillus.

21. The method of claim 18 , wherein the lymph node drainage areas comprise at least one of subiliac, popliteal, retropharangeal, superficial cervical, and axillary.

22. The method of claim 18 , wherein the animals comprise bovine, equine, ovine, porcine, or caprine.

23. The method of claim 18 , wherein the bacteria is selected from Salmonella Newport and Montevideo.

Assignees

• Univ Texas Tech System
• United States Dept Of Agriculture

Inventors

• Loneragan Guy
• Edrington Thomas
• Malin Katelyn

Classifications

• C12Q1/18 Primary

  Testing for antimicrobial activity of a material · CPC title

• G01N2800/52

  Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis · CPC title

• G01N2800/26

  Infectious diseases, e.g. generalised sepsis · CPC title

• G01N2333/255

  Salmonella (G) · CPC title

• A61K39/0275 Primary

  Salmonella · CPC title