Parental RNAi suppression of chromatin remodeling genes to control coleopteran pests

Patent metadata

Field	Value
Publication number	US-9994844-B2
Application number	US-201514971188-A
Country	US
Kind code	B2
Filing date	Dec 16, 2015
Priority date	Oct 6, 2011
Publication date	Jun 12, 2018
Grant date	Jun 12, 2018

Abstract

This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

First claim

What may be claimed is:

1. An isolated nucleic acid molecule comprising a polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide comprises SEQ ID NO:8, the complement of SEQ ID NO:8, SEQ ID NO:10, or the complement of SEQ ID NO:10.

2. The nucleic acid molecule of claim 1 , wherein the polynucleotide is comprised in a vector.

3. A ribonucleic acid (RNA) molecule encoded by the nucleic acid molecule of claim 1 .

4. The RNA molecule of claim 3 , wherein the molecule is a double-stranded ribonucleic acid (dsRNA) molecule.

5. The dsRNA molecule of claim 4 , wherein the dsRNA molecule is a hairpin RNA (hpRNA) molecule.

6. The RNA molecule of claim 3 , wherein the molecule is a single-stranded RNA molecule.

7. The nucleic acid molecule of claim 1 , wherein the heterologous promoter is functional in a plant cell.

8. A prokarvotic cell comprising the nucleic acid molecule of claim 1 .

9. A eukaryotic cell comprising the nucleic acid molecule of claim 1 .

10. A plant cell comprising the nucleic acid molecule of claim 7 .

11. A transgenic plant comprising the nucleic acid molecule of claim 7 .

12. A transgenic plant part comprising the nucleic acid molecule of claim 7 .

13. A commodity product produced from the transgenic plant of claim 11 , wherein the commodity product comprises a detectable amount of the polynucleotide.

14. The transgenic plant of claim 11 , wherein a cell of the plant comprises a double-stranded ribonucleic acid (dsRNA) molecule encoded by the polynucleotide.

15. The cell of claim 10 , wherein the cell is a Zea mays cell.

16. The transgenic plant of claim 11 , wherein the plant is Zea mays.

17. The transgenic plant of claim 11 , wherein the dsRNA molecule functions to inhibit a biological function within a Diabrotica virgifera pest when the Diabrotica virgifera pest ingests a part of the plant.

18. A method for controlling a Diabrotica virgifera pest population, the method comprising contacting a pest of the population with the dsRNA molecule of claim 4 .

19. The method according to claim 18 , wherein the dsRNA molecule is a hairpin RNA (hpRNA) molecule.

20. The method according to claim 18 , wherein the dsRNA molecule is introduced into a male Diabrotica virgifera pest.

21. The method according to claim 18 , wherein the dsRNA molecule is introduced into a female Diabrotica virgifera pest, the method further comprising releasing the female Diabrotica virgifera pest comprising the dsRNA molecule into the pest population, wherein mating between the female Diabrotica virgifera pest and male pests of the population produces fewer viable offspring than mating between other female pests and male pests of the population.

22. A method for controlling a Diabrotica virgifera pest population, the method comprising:
feeding the plant cell of claim 10 to a Diabrotica virgifera pest of the population, wherein the polynucleotide is expressed in the plant cell to produce a dsRNA molecule, functioning when ingested by the Diabrotica virgifera pest to inhibit a biological function of the Diabrotica virgifera pest or pest population.

23. A method of controlling Diabrotica virgifera pest infestation in a plant, the method comprising providing in the diet of a Diabrotica virgifera pest a double-stranded ribonucleic acid (dsRNA) molecule comprising as one strand a polyribonucleotide that is specifically hybridizable with SEQ ID NO:139, the complement of SEQ ID NO:139, SEQ ID NO: 140, or the complement of SEQ ID NO: 140.

24. The method according to claim 23 , wherein the dsRNA molecule is a hairpin RNA (hpRNA) molecule, and the diet comprises a plant cell that expresses the hpRNA molecule.

25. A method for producing a transgenic corn plant, the method comprising:
introducing the nucleic acid molecule of claim 7 into a corn plant to produce a transgenic corn plant, wherein expression of the polynucleotide produces a dsRNA molecule that functions upon contact with a Diabrotica virgifera pest to inhibit a biological function within the pest when the pest ingests a portion of the corn plant.

26. The nucleic acid molecule of claim 1 , further comprising a polynucleotide encoding a polypeptide from Bacillus thuringiensis.

27. The nucleic acid molecule of claim 26 , wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1B, Cry1I, Cry2A, Cry3, Cry7A, Cry8, Cry9D, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1A, and Cyt2C.

28. The plant cell of claim 10 , wherein the cell comprises a polynucleotide encoding a polypeptide from Bacillus thuringiensis.

29. The plant cell of claim 28 , wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1B, Cry1I, Cry2A, Cry3, Cry7A, Cry8, Cry9D, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, CytlA, and Cyt2C.

30. The transgenic plant of claim 11 , wherein the plant comprises a polynucleotide encoding a polypeptide from Bacillus thuringiensis.

31. The transgenic plant of claim 30 , wherein the polypeptide from B. thuringiensis is selected from a group comprising Cry1B, Cry1I, Cry2A, Cry3, Cry7A, Cry8, Cry9D, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1A, and Cyt2C.

32. The method according to claim 23 , wherein the method further comprises feeding the Diabrotica virgifera pest with an insecticidal polypeptide from Bacillus thuringiensis.

33. The method according to claim 32 , wherein the insecticidal polypeptide from B. thuringiensis is selected from a group comprising Cry1B, Cry1I, Cry2A, Cry3, Cry7A, Cry8, Cry9D, Cry14, Cry18, Cry22, Cry23, Cry34, Cry35, Cry36, Cry37, Cry43, Cry55, Cyt1A, and Cyt2C.

34. The transgenic plant part of claim 12 , wherein the plant part is a seed.

35. The method according to claim 18 , wherein contacting the Diabrotica virgifera pest with the dsRNA molecule comprises contacting the pest with a sprayable composition comprising the agent.

36. The method according to claim 18 , wherein contacting the Diabrotica virgifera pest with the dsRNA molecule comprises feeding a plant cell comprising the dsRNA molecule to the pest.

37. The method according to claim 25 , the method further comprising cultivating the corn plant.

38. The method according to claim 22 , wherein feeding the plant cell to the Diabrotica virgifera of the population comprises providing a transgenic plant comprising the plant cell to the coleopteran pest population.

Assignees

• Dow Agrosciences Llc
• Univ Nebraska

Inventors

• Siegfried Blair D
• Narva Kenneth E
• Arora Kanika
• Worden Sarah E
• Khajuria Chitvan
• Fishilevich Elane
• Storer Nicholas P
• Frey Meghan
• Hamm Ronda L
• Velez Ana M

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8286

  for insect resistance · CPC title

• A01N57/16

  containing heterocyclic radicals · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title