Recombinant host cell expressing a family glycoside hydrolase 61 polypeptide

Patent metadata

Field	Value
Publication number	US-9994832-B2
Application number	US-201615199061-A
Country	US
Kind code	B2
Filing date	Jun 30, 2016
Priority date	Mar 31, 2011
Publication date	Jun 12, 2018
Grant date	Jun 12, 2018

Abstract

The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material.

First claim

What is claimed is:

1. An isolated recombinant host cell transformed with a nucleic acid construct comprising a polynucleotide encoding a Family 61 glycosyl hydrolase (GH61) polypeptide having cellulolytic enhancing activity and one or more heterologous control sequences that direct the production of the GH61 polypeptide, wherein the polynucleotide is operably linked to the one or more heterologous control sequences that direct the production of the GH61 polypeptide, and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
(a) a GH61 polypeptide comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence of amino acids 22 to 320 of SEQ ID NO: 2;
(b) a fragment of the GH61 polypeptide of (a), wherein the fragment has GH61 cellulolytic enhancing activity.

2. The recombinant host cell of claim 1 , wherein the GH61 polypeptide having GH61 cellulolytic enhancing activity comprises (i) the amino acid sequence of SEQ ID NO: 2; (ii) the amino acid sequence of amino acids 22 to 320 of the GH61 polypeptide of SEQ ID NO: 2; or (iii) a fragment of (i) or (ii), wherein the fragment has cellulolytic enhancing activity.

3. The recombinant host cell of claim 1 , wherein the GH61 polypeptide having cellulolytic enhancing activity is encoded by (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1, or (ii) a polynucleotide comprising the nucleotide sequence of nucleotides 64 to 1018 or nucleotides 64 to 101 and 160 to 1018 of SEQ ID NO: 1.

4. A method of producing a GH61 polypeptide having cellulolytic enhancing activity, comprising cultivating the recombinant host cell of claim 1 under conditions conducive for production of the GH61 polypeptide, thereby producing the GH61 polypeptide.

5. The method of claim 4 , wherein the GH61 polypeptide having GH61 cellulolytic enhancing activity comprises (i) the amino acid sequence of SEQ ID NO: 2; (ii) the amino acid sequence of amino acids 22 to 320 of the GH61 polypeptide of SEQ ID NO: 2; or (iii) a fragment of (i) or (ii), wherein the fragment has cellulolytic enhancing activity.

6. The method of claim 4 , wherein the GH61 polypeptide having GH61 cellulolytic enhancing activity comprises the amino acid sequence of amino acids 22 to 320 of the GH61 polypeptide of SEQ ID NO: 2.

7. The method of claim 4 , wherein the GH61 polypeptide having cellulolytic enhancing activity is encoded by (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1, or (ii) a polynucleotide comprising the nucleotide sequence of nucleotides 64 to 1018 or nucleotides 64 to 101 and 160 to 1018 of SEQ ID NO: 1.

Assignees

• Novozymes Inc

Inventors

• Schnorr Kirk
• Kramer Randall

Classifications

• C12N9/2437

  Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12P19/12

  Disaccharides · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title