Anti-human transferrin receptor antibody that passes through blood-brain barrier

Patent metadata

Field	Value
Publication number	US-9994641-B2
Application number	US-201415107839-A
Country	US
Kind code	B2
Filing date	Dec 24, 2014
Priority date	Dec 25, 2013
Publication date	Jun 12, 2018
Grant date	Jun 12, 2018

Abstract

Disclosed are a novel anti-human transferrin receptor antibody comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 5, 6 and 7 that can pass through the blood-brain barrier, a fusion protein comprising a protein necessary to be brought into function in the central nervous system and the antibody, and method of their production. The fusion protein comprises amino acid sequences of an anti-human transferrin receptor antibody that recognizes an amino acid sequence selected from the group consisting of SEQ ID NOs:1, 2, and 3, and of other protein that is bound thereto on the C-terminal side.

First claim

The invention claimed is:

1. A single-chain anti-human transferrin receptor antibody comprising an amino acid sequence selected from the group consisting of SEQ ID Nos: 4, 5, 6, and 7.

2. A DNA sequence encoding the antibody according to claim 1 .

3. A fusion protein comprising the antibody according to claim 1 and another protein bound thereto on the C-terminal side.

4. The fusion protein according to claim 3 wherein the another protein is bound to the C-terminal side of the antibody by a linker sequence consisting of 3-50 amino acids.

5. The fusion protein according to claim 3 , wherein said another protein is a lysosomal enzyme.

6. The fusion protein according to claim 5 , wherein the lysosomal enzyme is human iduronate 2-sulfatase.

7. A DNA sequence encoding the fusion protein according to claim 3 .

8. A mammalian expression vector comprising the DNA sequence according to claim 7 .

9. A mammalian cell transformed with the mammalian expression vector according to claim 8 .

10. The cell according to claim 9 , wherein the mammalian cell is a CHO cell.

Assignees

• Japan Chem Res

Inventors

• Sonoda Hiroyuki
• Morimoto Hideto
• Koshimura Yuri
• Kinoshita Masafumi
• Takagi Haruna
• Yoshii Yoshiko

Classifications

• C12Y301/06013

  Iduronate-2-sulfatase (3.1.6.13) · CPC title

• C07K2317/21

  from primates, e.g. man · CPC title

• C07K16/2881 Primary

  against CD71 · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K2317/622

  Single chain antibody (scFv) · CPC title