Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Patent metadata

Field	Value
Publication number	US-9988657-B2
Application number	US-201514631962-A
Country	US
Kind code	B2
Filing date	Feb 26, 2015
Priority date	Feb 27, 2014
Publication date	Jun 5, 2018
Grant date	Jun 5, 2018

Abstract

A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

First claim

What is claimed is:

1. A method that comprises:
(a) providing a glucan synthesis reaction;
(b) obtaining a fraction of the glucan synthesis reaction that comprises a saccharide byproduct of the glucan synthesis reaction, wherein the saccharide byproduct is a disaccharide or oligosaccharide and comprises at least one alpha-1,3 glucosyl-glucose linkage; and
(c) contacting the saccharide byproduct in the fraction with an alpha-glucosidase enzyme, wherein the alpha-glucosidase enzyme hydrolyzes at least one alpha-1,3 glucosyl-glucose linkage of the saccharide byproduct.

2. The method of claim 1 , wherein the alpha-glucosidase enzyme is immobilized.

3. The method of claim 1 , wherein the degree of polymerization of the saccharide byproduct before hydrolysis is 3 to 7.

4. The method of claim 1 , wherein the alpha-glucosidase enzyme is a transglucosidase.

Assignees

• Du Pont

Inventors

• Nagy Kevin D
• Hago Erwin Columbus
• Shetty Jayarama K
• Hennessey Susan Marie
• Dicosimo Robert
• Hua Ling
• Ramirez Rodrigo
• Tang Zhongmei
• Yu Zheyong

Classifications

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P19/18

  produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title

• C12P19/16

  produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin (non-biological hydrolysis of starch C08B30/00) · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title