Composition including pint gene expression inhibitor or pint activity inhibitor and use thereof

Patent metadata

Field	Value
Publication number	US-9988632-B2
Application number	US-201615378368-A
Country	US
Kind code	B2
Filing date	Dec 14, 2016
Priority date	Dec 24, 2015
Publication date	Jun 5, 2018
Grant date	Jun 5, 2018

Abstract

Provided are a composition for stimulating DNA damage in cells, the composition including a p53 induced non-coding transcript (PINT) gene expression inhibitor or a PINT activity inhibitor, a method of killing senescent cells in a subject, and a method of monitoring DNA damage in cells.

First claim

What is claimed is:

1. A composition comprising one or more of an expression inhibitor inhibiting p53 induced non-coding transcript (PINT) gene expression or an activity inhibitor inhibiting PINT activity, wherein the expression inhibitor or activity inhibitor comprises an siRNA that comprises a pair of nucleotide sequences selected from the group consisting of SEQ ID NO: 12 and SEQ ID NO: 13; SEQ ID NO: 16 and SEQ ID NO: 17; and SEQ ID NO: 18 and SEQ ID NO: 19.

2. The composition of claim 1 , further comprising a pharmaceutically acceptable carrier.

3. A method of killing senescent cells in a subject, the method comprising inhibiting p53-induced non-coding transcript (PINT) gene expression or inhibiting PINT activity in the senescent cells by administering to the cells the composition of claim 1 .

4. The method of claim 3 , wherein the PINT expression inhibitor and/or the PINT activity inhibitor is administered with a pharmaceutically acceptable carrier.

5. The method of claim 3 , wherein the composition is topically administered to a senescent site.

6. The method of claim 5 , wherein the senescent site is a site on the skin which has wrinkles, freckles, pigmentation spots, or a combination thereof.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Choi Hyojei
• Kim Myoungsoon
• Lee Youngsam

Classifications

• C12N2310/14

  interfering nucleic acids [NA] · CPC title

• C12Q1/6881

  for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N2320/30

  Special therapeutic applications · CPC title

• A61K8/606

  Nucleosides; Nucleotides; Nucleic acids · CPC title