Method of enzyme inactivation

Patent metadata

Field	Value
Publication number	US-9988621-B2
Application number	US-201214373029-A
Country	US
Kind code	B2
Filing date	Feb 15, 2012
Priority date	Feb 15, 2012
Publication date	Jun 5, 2018
Grant date	Jun 5, 2018

Abstract

The present invention relates to a method for the inactivation of enzymes with a composition comprising a sulfonated peroxycarboxylic acid according to formula (I) wherein: R 1 is hydrogen, or a substituted or unsubstituted C m alkyl group; R 2 is a substituted or unsubstituted C n alkyl group; X is a hydrogen atom, a cationic group, or an ester forming moiety; Y is a hydrogen atom, a cationic group, or an ester forming moiety; n is 1 to 10; m is 1 to 10; and n+m is less than or equal to 18, wherein the composition is contacted with the enzyme.

First claim

The invention claimed is:

1. A method for the inactivation of enzymes, said method comprising:
applying an enzyme-based cleaning composition comprising a lipase to a surface; and
applying an enzyme-inactivation composition to said surface, wherein said enzyme-inactivation composition comprises a sulfonated peroxycarboxylic acid according to formula 1:

wherein: R1 is hydrogen, or a substituted or unsubstituted Cm alkyl group; R2 is a substituted or unsubstituted Cn alkyl group; X is a hydrogen atom, a cationic group, or an ester forming moiety; Y is a hydrogen atom, a cationic group, or an ester forming moiety; n is 1 to 10; m is 1 to 10; and n+m is less than or equal to 18,
wherein said enzyme-inactivation composition is effective for inactivation of said lipase as determined by an ELISA analysis.

2. The method of claim 1 , wherein Y is a hydrogen atom.

3. The method of claim 1 , wherein said enzyme-inactivation composition further comprises an oxidizing reagent, wherein said oxidizing reagent is not a chlorine-containing compound.

4. The method of claim 1 , wherein said enzyme-inactivation composition further comprises hydrogen peroxide.

5. The method of claim 1 , wherein said enzyme-inactivation composition further comprises a C1 to C22 carboxylic or peroxycarboxylic acid.

6. The method of claim 1 , wherein said applying an enzyme-inactivation composition to said surface is carried out at a temperature in the range of 10° C. to 70° C.

7. The method of claim 1 , wherein said enzyme-inactivation composition has a pH of 7 to 9.

8. The method of claim 1 , wherein said enzyme-inactivation composition further comprises:
10 to 20 wt-% sulfonated oleic acid;
up to 10 wt-% acetic acid;
5 to 10 wt-% peracetic acid;
1 to 5 wt-% octanoic acid;
1 to 5 wt-% peroxyoctanoic acid;
1 to 5 wt-% hydroxyethane diphosphonic acid; and
8 to 35 wt-% hydrogen peroxide.

9. The method of claim 1 , wherein said enzyme-inactivation composition of formula 1 is selected from 11-sulfo-undecanoic acid, 10,11-disulfo-undecanoic acid, sulfonated oleic acid, sulfonated linoleic acid, sulfonate palmitoleic acid, and sulfonated stearic acid.

10. The method of claim 1 , wherein said surface comprises a surface on process equipment.

11. The method of claim 10 , wherein said surface comprises a food or beverage preparation surface.

12. The method of claim 1 , wherein said surface comprises a fabric or textile.

13. The method of claim 1 , wherein said surface comprises a membrane.

14. The method of claim 1 , wherein applying said enzyme-based cleaning composition comprises a clean-in-place procedure.

15. The method of claim 1 , further comprising rinsing said surface after applying the enzyme-based cleaning composition to said surface.

16. The method of claim 1 , wherein said enzyme-inactivation composition is effective for inactivating enzymes at room temperature.

17. The method of claim 1 , wherein said enzyme-inactivation composition is applied to said surface at room temperature.

Assignees

• Patten Anja
• Nolde Christof
• Vogt Petra
• Ecolab Usa Inc

Inventors

• Patten Anja
• Nolde Christof
• Vogt Petra

Classifications

• C11D3/386

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title

• C12N9/99 Primary

  Enzyme inactivation by chemical treatment · CPC title

• B01D65/02

  Membrane cleaning or sterilisation {; Membrane regeneration} · CPC title

• C12Y301/01 Primary

  Carboxylic ester hydrolases (3.1.1) · CPC title

• B01D2321/166

  Use of enzymatic agents · CPC title