Polypeptides having xanthan degrading activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9988616-B2
Application number	US-201615245759-A
Country	US
Kind code	B2
Filing date	Aug 24, 2016
Priority date	May 7, 2012
Publication date	Jun 5, 2018
Grant date	Jun 5, 2018

Abstract

The present invention relates to isolated polypeptides having xanthan degrading activity, catalytic domains and polynucleotides encoding the polypeptides and catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and catalytic domains.

First claim

What is claimed is:

1. A composition comprising a GH9 endonuclease and a polypeptide having at least 90% sequence identity to the mature form of SEQ ID NO: 64, wherein said GH9 endonuclease has increased activity on xanthan gum in the presence of said xanthan lyase compared to in its absence.

2. The composition of claim 1 , which is a detergent composition comprising one or more detergent components.

3. A method for degrading xanthan gum comprising applying a composition comprising claim 1 to the xanthan gum.

4. The method of claim 3 , wherein the xanthan gum is on the surface of a textile or hard surface, such as dish wash.

5. The method of claim 3 , wherein the xanthan gum is used in fracturing of a subterranean formation perpetrated by a well bore.

6. The method of claim 3 , wherein the xanthan gum is a component in borehole filtercake.

7. An isolated polynucleotide encoding a polypeptide having at least 90% sequence identity to the mature form of SEQ ID NO: 64, wherein said polynucleotide is operably linked to a heterologous control sequence that directs production of the polypeptide in a host cell.

8. An nucleic acid construct or expression vector comprising the polynucleotide of claim 7 .

9. A recombinant host cell comprising the polynucleotide of claim 7 .

10. A method of producing a xanthan lyase, comprising: (a) cultivating a cell of claim 9 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

11. A method for degrading xanthan gum comprising pretreating xanthan gum with a xanthan lyase having at least 90% sequence identity to SEQ ID NO: 64 and then degrading said xanthan gum by applying a GH9 endonuclease.

12. The method of claim 11 , wherein the xanthan gum is on the surface of a textile or hard surface, such as dish wash.

13. The method of claim 11 , wherein the xanthan gum is used in fracturing of a subterranean formation perpetrated by a well bore.

14. The method of claim 11 , wherein the xanthan gum is a component in borehole filtercake.

Assignees

• Novozymes As

Inventors

• Segura Dorotea Raventos
• Halin Peter Fischer
• Viksoe-Nielsen Anders
• Anderson Lars
• Borchert Martin Simon
• Murphy Leigh
• Boisen Astrid
• Palmen Lorena G
• Jensen Kenneth
• Sjoeholm Carsten
• Hoff Tine
• Blom Charlotte

Classifications

• C12Y402/02012

  Xanthan lyase (4.2.2.12) · CPC title

• C11D3/38636

  containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• C09K8/02

  Well-drilling compositions · CPC title

• C09K8/52

  Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning · CPC title