Method and device for assigning a blood plasma sample

Patent metadata

Field	Value
Publication number	US-9983192-B2
Application number	US-201615234511-A
Country	US
Kind code	B2
Filing date	Aug 11, 2016
Priority date	Feb 19, 2014
Publication date	May 29, 2018
Grant date	May 29, 2018

Abstract

A device and method for assigning a blood plasma sample to a class from a predetermined set of classes are presented. The set of classes comprises a good class, a lipemic class, a hemolytic class and an icteric class. For assignment to one of the classes, the blood plasma sample is exposed to light and measurement values dependent on transmitted or scattered light power are evaluated in order to carry out an assignment.

First claim

We claim:

1. A method for assigning a blood plasma sample to a class from a predetermined set of classes, the blood plasma sample being contained in an at least partially transparent vessel, the method comprising:
exposing the blood plasma sample to light, the spectral composition of which comprises at least wavelengths from a predetermined set of wavelengths;
forming a wavelength-specific measurement value for a respective wavelength from the set of wavelengths, a respective measurement value being dependent on a light power transmitted through the blood plasma sample and the vessel at the respective wavelength; and
assigning the blood plasma sample to a class as a function of the wavelength-specific measurement values, wherein the predetermined set of classes comprises a hemolytic class, wherein the set of wavelengths comprises a first hemolytic wavelength in the range of from 535 nm to 547 nm and a second hemolytic wavelength in the range of from 510 nm to 520 nm and the blood plasma sample is assigned to the hemolytic class if a ratio of the measurement value at the first hemolytic wavelength and the measurement value at the second hemolytic wavelength is less than a first relative hemolytic threshold value.

2. The method according to claim 1 , wherein the first hemolytic wavelength is 541 nm.

3. The method according to claim 1 , wherein the second hemolytic wavelength is 515 nm.

4. The method according to claim 1 , wherein the predetermined set of classes comprises a lipemic class, the set of wavelengths comprises a lipemic wavelength in the range of from 610 nm to 700 nm and the blood plasma sample is assigned to the lipemic class if the measurement value at the lipemic wavelength is less than a lipemic threshold value.

5. The method according to claim 4 , wherein the lipemic wavelength is 650 nm or 685 nm.

6. The method according to claim 1 , wherein the predetermined set of classes comprises a lipemic class and a scattering measurement value is formed, which is dependent on a light power scattered by the blood plasma sample, the blood plasma sample being assigned to the lipemic class if the scattering measurement value is greater than a lipemic scattering threshold value.

7. The method according to claim 1 , wherein the blood plasma sample is assigned to the hemolytic class if the measurement value at the first hemolytic wavelength is less than an absolute hemolytic threshold value and if the blood plasma sample is not assigned to a lipemic class.

8. The method according to claim 1 , wherein the set of wavelengths comprises a third hemolytic wavelength in the range of from 610 nm to 700 nm and the blood plasma sample is assigned to the hemolytic class if a ratio of the measurement value at the first hemolytic wavelength and the measurement value at the third hemolytic wavelength is less than a second relative hemolytic threshold value.

9. The method according to claim 8 , wherein the third hemolytic wavelength is 650 nm or 685 nm.

10. The method according to claim 1 , wherein the predetermined set of classes comprises an icteric class, the set of wavelengths comprises a first icteric wavelength in the range of from 450 nm to 485 nm, and the blood plasma sample is assigned to the icteric class if the measurement value at the first icteric wavelength is less than an absolute icteric threshold value and if the blood plasma sample is not assigned to a lipemic class and is not assigned to a hemolytic class.

11. The method according to claim 10 , wherein the first icteric wavelength is 470 nm.

12. The method according to claim 1 , wherein the predetermined set of classes comprises an icteric class, the set of wavelengths comprises a first icteric wavelength in the range of from 450 nm to 485 nm and a second icteric wavelength in the range of from 510 nm to 520 nm, and the blood plasma sample is assigned to the icteric class if a ratio of the measurement value at the first icteric wavelength and the measurement value at the second icteric wavelength is less than a first relative icteric threshold value.

13. The method according to claim 12 , wherein the second icteric wavelength is 515 nm.

14. The method according to claim 1 , wherein the predetermined set of classes comprises a good class, and the blood plasma sample is assigned to the good class if the blood plasma sample is not assigned to a lipemic class, is not assigned to a hemolytic class and is not assigned to an icteric class.

15. The method according to claim 1 , wherein an error message is emitted if the blood plasma sample is assigned to at least two different classes.

16. The method according to claim 1 , further comprising,
detecting whether, and at what position, there is a label on the vessel, a number of threshold values being modified as a function thereof.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Klinec Darko
• Koehler Michael

Classifications

• G01N21/31

  Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} · CPC title

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title