Process for preparation of sugars and syrups

Patent metadata

Field	Value
Publication number	US-9982282-B2
Application number	US-201414782642-A
Country	US
Kind code	B2
Filing date	Apr 10, 2014
Priority date	Apr 10, 2013
Publication date	May 29, 2018
Grant date	May 29, 2018

Abstract

The present invention relates to a process for producing a syrup comprising liquefying an aqueous granular starch slurry with an alpha-amylase variant comprising an alteration at one or more positions corresponding to any of positions 1, 2, 68, 71, 126, 133, 142, 144, 156, 158, 176, 185, 201, 205, 213, 239, 279, 316, 318, 360, 416, 437 and 450 of SEQ ID NO: 1 to provide liquefied starch-containing material; saccharifying the liquefied starch-containing material in the presence of a glucoamylase, and a pullulanase derived from Bacillus deramificans, Bacillus subtilis, Bacillus amyloderamicans , or Bacillus acidopullulyticus to provide a dextrose syrup, and optionally isomerizing to provide a fructose syrup.

First claim

The invention claimed is:

1. A process for producing a syrup comprising:
a) liquefying an aqueous granular starch slurry with an alpha-amylase variant comprising a substitution at a position corresponding to one or more of positions 176, 185, 360 and/or 437 of SEQ ID NO: 1 to provide liquefied starch-containing material;
b) saccharifying the liquefied starch-containing material in the presence of a glucoamylase, and a pullulanase derived from Bacillus deramificans, Bacillus subtilis, Bacillus amyloderamificans , or Bacillus acidopullulyticus and an alpha-amylase variant comprising an alteration at one or more positions corresponding to any of positions 1, 2, 68, 71, 126, 133, 142, 144, 156, 158, 176, 185, 201, 205, 213, 239, 279, 316, 318, 360, 416, 437 and 450 of SEQ ID NO: 1 to provide a dextrose syrup, and optionally
c) isomerizing to provide a fructose syrup.

2. The process of claim 1 , wherein the alpha-amylase variant is added in an amount of about 0.0001-3 mg enzyme protein per gram dry solids.

3. The process of claim 1 , wherein the alpha-amylase variant comprises a substitution at position 176 and/or 185 of SEQ ID NO: 1.

4. The process of claim 1 , wherein the pullulanase is derived from Bacillus deramificans.

5. The process of claim 1 , wherein the starch slurry has 20-55% dry solids granular starch.

6. The process of claim 1 , wherein at least 85% of the dry solids of the granular starch is converted into a syrup, e.g., a dextrose syrup or a fructose syrup.

7. The process of claim 1 , wherein the granular starch is obtained from tubers, roots, stems, or whole grain.

8. The process of claim 1 , wherein the granular starch is obtained from cereals.

9. The process of claim 1 , wherein the granular starch is obtained from corn, cobs, wheat, barley, rye, milo, sago, cassava, tapioca, sorghum, rice or potatoes.

10. The process of claim 1 , wherein the granular starch is obtained from dry milling of whole grain or from wet milling of whole grain.

11. The process of claim 1 , wherein addition of the alpha-amylase variant in step a), step b), or a combination thereof results in higher DP1 product compared to a similar reaction without the alpha-amylase variant.

12. The process of claim 1 , wherein addition of the alpha-amylase variant in step a), step b), or a combination thereof results in reduced DP4+product compared to a similar reaction without the alpha-amylase variant.

13. The process of claim 1 , wherein addition of the alpha-amylase variant in step a), step b), or a combination thereof results in reduced DP2 product compared to a similar reaction without the alpha-amylase variant.

Assignees

• Novozymes As

Inventors

• Elder Michael
• Deinhammer Randall
• Cui Xiaoyuan

Classifications

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C13K11/00

  Fructose (separation from invert sugar C13K3/00) · CPC title

• C13K1/06

  obtained by saccharification of starch or raw materials containing starch · CPC title

• C12P19/24

  produced by the action of an isomerase, e.g. fructose · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title