Method of creating spray device for sheathless CE-MS, spray device for sheathless CE-MS, and sheathless CE-MS device

Patent metadata

Field	Value
Publication number	US-9978573-B2
Application number	US-201615552918-A
Country	US
Kind code	B2
Filing date	Mar 15, 2016
Priority date	Apr 1, 2015
Publication date	May 22, 2018
Grant date	May 22, 2018

Abstract

Processing a capillary distal end into acute-angle, an electrophoretic liquid passing hole through which electrophoretic liquid pass is opened in a flexible insulating plate. An electrodialysis-membrane is bonded covering the electrophoretic liquid passing hole; the capillary is securely bonded to the insulating plate portion with no gap therebetween, the portion excluding electrophoretic liquid passing hole on the electrodialysis top-membrane. A crack forms at the capillary portion at the electrophoretic liquid passing hole, with the capillary entirely secured to the insulating plate except portion at the electrophoretic liquid passing hole. The capillary is securely bonded to the insulating plate; reservoir stores electrophoretic liquid on the insulating plate-side to which the capillary is unsecured. An electrode insertion hole into which an electrode is inserted opened in the reservoir upper portion; the electrode is securely inserted into the electrode insertion hole. By this, high-sensitivity measurement is using a spray device for sheathless CE-MS.

First claim

The invention claimed is:

1. A method of creating a spray device for sheathless CE-MS, the method comprising:
a step of processing a distal end of a capillary to have an acute angle;
a step of opening an electrophoretic liquid passing hole, through which an electrophoretic liquid can pass, in a flexible insulating plate;
a step of bonding an electrodialysis membrane so as to cover the electrophoretic liquid passing hole;
a step of securely bonding the capillary to a portion of the insulating plate with no gap therebetween, the portion excluding the electrophoretic liquid passing hole on top of the electrodialysis membrane;
a step of forming a crack at a portion of the capillary at the electrophoretic liquid passing hole, the capillary being entirely secured to the insulating plate except a portion at the electrophoretic liquid passing hole;
a step of entirely securely bonding the capillary to the insulating plate;
a step of placing a reservoir for storing the electrophoretic liquid on a side of the insulating plate to which the capillary is not secured;
a step of opening an electrode insertion hole, into which an electrode is inserted, in an upper portion of the reservoir; and
a step of inserting and securing the electrode into the electrode insertion hole.

2. The method of creating a spray device for sheathless CE-MS according to claim 1 , wherein
the step of forming the crack includes:
a step of scratching, with a cutter, a surface of the portion of the capillary at the electrophoretic liquid passing hole; and
a step of deflecting the insulating plate to bend the capillary, thereby forming a crack at the portion at the electrophoretic liquid passing hole.

3. The method of creating a spray device for sheathless CE-MS according to claim 1 , wherein the insulating plate is a plastic plate or a glass plate.

4. The method of creating a spray device for sheathless CE-MS according to claim 1 , wherein the electrodialysis membrane is an ion exchange membrane.

5. The method of creating a spray device for sheathless CE-MS according to claim 1 , wherein the reservoir is made of an insulator.

6. The method of creating a spray device for sheathless CE-MS according to claim 2 , wherein the cutter is a ceramic cutter.

7. A spray device for sheathless CE-MS, the spray device comprising:
a capillary with a distal end processed to have an acute angle and with a crack formed at an intermediate portion;
an insulating plate to which the capillary is securely bonded and in which an electrophoretic liquid passing hole for enabling an electrophoretic liquid to pass therethrough is formed at a portion having the crack formed;
an electrodialysis membrane bonded onto the electrophoretic liquid passing hole of the insulating plate;
a reservoir which is placed on a side of the insulating plate having the capillary not secured thereto and which stores the electrophoretic liquid; and
an electrode which is inserted and secured to an upper portion of the reservoir.

8. A sheathless CE-MS device comprising the spray device according to claim 7 .

9. The method of creating a spray device for sheathless CE-MS according to claim 2 , wherein the insulating plate is a plastic plate or a glass plate.

Assignees

• Univ Keio

Inventors

• Soga Tomoyoshi
• Hirayama Akiyoshi
• Abe Hiroshi

Classifications

• H01J49/045 Primary

  with means for using a nebulising gas, i.e. pneumatically assisted · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title

• B01D61/422

  Electrodialysis · CPC title

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• G01N27/4473 Primary

  by electric means · CPC title