Staggered chromatography mass spectrometry

Patent metadata

Field	Value
Publication number	US-9976995-B2
Application number	US-201515317295-A
Country	US
Kind code	B2
Filing date	Jun 12, 2015
Priority date	Jun 12, 2014
Publication date	May 22, 2018
Grant date	May 22, 2018

Abstract

An analytical instrument is disclosed comprising a liquid chromatography system comprising a first column, a chromatographic delay line and a splitter arranged and adapted in a mode of operation to split eluent from the first column into a first portion of eluent and a second portion of eluent. The instrument further comprises a first device. The liquid chromatography system is arranged and adapted in the mode of operation: (i) to pass the first portion of eluent from the splitter to the first device such that a first part of the first portion of eluent arrives at the first device at a first time ti and a second part of the first portion of eluent arrives at the first device at a second time t 2 . The system is further arranged and adapted: (ii) to pass the second portion of eluent from the splitter through the chromatographic delay line to the first device such that the passage of the second portion of eluent from the splitter to the first device is delayed relative to the passage of the first portion of eluent from the splitter to the first device, and such that a first part of the second portion of eluent arrives at the first device at a third time t 3 , where t 1 <t 3 <t 2 . The analytical instrument is arranged and adapted to analyze the first part of the first portion of eluent in a first mode of operation and to analyze the first part of the second portion of eluent in a second mode of operation.

First claim

The invention claimed is:

1. An analytical instrument comprising:
a liquid chromatography system comprising a first column, a chromatographic delay line and a splitter arranged and adapted in a mode of operation to split eluent from said first column into a first portion of eluent and a second portion of eluent; and
at least one first device;
wherein said liquid chromatography system is arranged and adapted in said mode of operation: (i) to pass said first portion of eluent from said splitter to said first device such that a first part of said first portion of eluent arrives at said first device at a first time t 1 and a second part of said first portion of eluent arrives at said first device at a second time t 2 ; and (ii) to pass said second portion of eluent from said splitter through said chromatographic delay line to said first device, such that the passage of said second portion of eluent from said splitter to said first device is delayed relative to the passage of said first portion of eluent from said splitter to said first device, and such that a first part of said second portion of eluent arrives at said first device at a third time t 3 , where t 1 <t 3 <t 2 ;
wherein said analytical instrument is arranged and adapted:
to analyse said first part of said first portion of eluent in a first mode of operation; and
to analyse said first part of said second portion of eluent in a second mode of operation.

2. An analytical instrument as claimed in claim 1 , wherein said system is arranged and adapted such that the time Δt 1 taken for said first portion of eluent to pass from said splitter to said first device is less than the time Δt 2 taken for said second portion of eluent to pass from said splitter to said first device.

3. An analytical instrument as claimed in claim 2 , wherein the delay time ΔT=Δt 2 −Δt 1 is fixed or variable.

4. An analytical instrument as claimed in claim 1 , wherein said first part of said first portion of eluent and said first part of said second portion of eluent comprise eluent eluted from said first column at the same retention time.

5. An analytical instrument as claimed in claim 1 , wherein:
said first part of said first portion of eluent comprises a first chromatographic peak of said first portion of eluent;
said second part of said first portion of eluent comprises a second chromatographic peak of said first portion of eluent;
said first part of said second portion of eluent comprises a third chromatographic peak of said second portion of eluent; and
said first chromatographic peak and said third chromatographic peak are corresponding chromatographic peaks from the same chromatographic peak of said eluent.

6. An analytical instrument as claimed in claim 5 , wherein said analytical instrument is arranged and adapted to select said third time t 3 such that the part of said first portion of eluent that arrives at said first device at said third time t 3 comprises no chromatographic peaks or comprises relatively unimportant, uninteresting and/or low intensity chromatographic peaks.

7. An analytical instrument as claimed in claim 1 , wherein:
said system is arranged and adapted such that the path length Δd 1 for said first portion of eluent to pass from said splitter to said first device is shorter than the path length Δd 2 for said second portion of eluent to pass from said splitter to said first device; and/or
said system is arranged and adapted such that the average speed or velocity Δv 1 of said first portion of eluent as it passes from said splitter to said first device is greater than the average speed or velocity Δv 2 of said second portion of eluent as it passes from said splitter to said first device.

8. An analytical instrument as claimed in claim 1 , wherein said system is arranged and adapted such that said eluent flows continuously as it passes from said first column to said first device.

9. An analytical instrument as claimed in claim 1 , wherein said system is arranged and adapted in a further mode of operation to pass substantially all eluent from said first column to said first device without passing said eluent through said chromatographic delay line.

10. An analytical instrument as claimed in claim 9 , wherein said system is arranged and adapted to switch from said further mode of operation to said splitting mode of operation upon said instrument determining, analysing, measuring, detecting, predicting or estimating that one or more analytes, components or chromatographic peaks of interest are emerging, eluting or being transmitted from said first column.

11. An analytical instrument as claimed in claim 9 , wherein said first device comprises an ion source or wherein said instrument further comprises an ion source; and
wherein said liquid chromatography system is arranged and adapted to switch from said further mode of operation to said splitting mode of operation upon said instrument determining, analysing, measuring, detecting, predicting or estimating that one or more analytes, components or chromatographic peaks of interest are being eluted to said ion source and/or that ions of interest are being emitted from said ion source.

12. An analytical instrument as claimed in claim 9 , further comprising an ion detector;
wherein said liquid chromatography system is arranged and adapted to switch from said further mode of operation to said splitting mode of operation upon said ion detector detecting ions of interest.

13. An analytical instrument as claimed in claim 1 , wherein:
said liquid chromatography system further comprises a fluid delivery system for delivering a fluid to said first column; and
wherein said system is arranged and adapted to alter or vary the flow rate at which fluid is passed through said first column upon said instrument determining, analysing, measuring, detecting, predicting or estimating that one or more analytes, components or chromatographic peaks of interest are emerging, eluting or being transmitted from said first column.

14. An analytical instrument as claimed in claim 1 , further comprising an analyser downstream of said first device, wherein said analyser is arranged and adapted:
to analyse said first part of said first portion of eluent in said first mode of operation; and
to analyse said first part of said second portion of eluent in said second mode of operation.

15. An analytical instrument as claimed in claim 14 , wherein said analyser comprises a chromatography detector, a mass spectrometer or an ion mobility spectrometer.

16. An analytical instrument as claimed in claim 1 , wherein said first and/or second mode of operation comprises:
a mass spectrometry (“MS”) mode of operation; a tandem mass spectrometry (“MS/MS”) mode of operation; a mode of operation in which parent or precursor ions are alternatively fragmented or reacted to produce fragment or product ions, and not fragmented or reacted or fragmented or reacted to a lesser degree; a Multiple Reaction Monitoring (“MRM”) mode of operation; a Data Dependent Analysis (“DDA”) mode of operation; a Data Independent Analysis (“DIA”) mode of operation; a Quantification mode of operation; or an Ion Mobility Spectrometry (“IMS”) mode of operation; and/or
(i) a Collisional Induced Dissociation (“CID”) mode of operation; (ii) a Surface Induced Dissociation (“SID”) mode of operation; (iii) an Electron Transfer Dissociation (“ETD”) mode of operation; (iv) an Electron Capture Dissociation (“ECD”) mode of operation; (v) an Electron Collision or Impact Dissociation mode of operation; (vi) a Photo Induced Dissociation (“PID”) mode of operation; (v

Assignees

• Micromass Ltd

Inventors

• Brown Jeffery
• Foster Steven

Classifications

• G01N30/7233 Primary

  interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title

• G01N30/62

  Detectors specially adapted therefor · CPC title

• B01D15/10 Primary

  characterised by constructional or operational features · CPC title

• G01N2030/027

  Liquid chromatography · CPC title

• G01N30/461

  with serial coupling of separation columns · CPC title