Accumulation of omega-7 fatty acids in plant seeds

Patent metadata

Field	Value
Publication number	US-9976155-B2
Application number	US-201615130788-A
Country	US
Kind code	B2
Filing date	Apr 15, 2016
Priority date	Jun 24, 2010
Publication date	May 22, 2018
Grant date	May 22, 2018

Abstract

Compositions and methods include genetically encoding and expressing a novel Δ 9 -18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel Δ 9 -18:0-ACP desaturase. In other embodiments, amino acid sequences have Δ 9 -18:0-ACP desaturase activity. Methods can involve expression of Δ 9 -18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

First claim

What is claimed is:

1. A method for increasing the amount of ω-7 fatty acids in plant material, the method comprising:
transforming plant material with a nucleic acid molecule comprising a polynucleotide that is at least 60% identical to SEQ ID NO:1, wherein the plant material comprises two means for increasing levels of 16:0-ACP in the plant material and an extraplastidial palmitic acid (16:0) desaturase, and wherein the polynucleotide encodes a Δ9 desaturase enzyme comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:2, such that the amount of ω-7 fatty acids in the plant material is increased,
thereby producing a transgenic plant material comprising each of:
the polynucleotide,
the Δ9 desaturase enzyme,
the two means for increasing levels of 16:0-ACP in the plant material, and
the extraplastidial palmitic acid (16:0) desaturase.

2. The method of claim 1 , wherein the polynucleotide encodes a plastidial delta-9 desaturase having at least 90% identity to SEQ ID NO:2.

3. The method of claim 1 , further comprising culturing the transformed plant material to obtain a plant.

4. The method of claim 1 , wherein the plant is selected from a genus selected from the group comprising Arabidopsis, Borago, Brassica, Ricinus, Theobroma, Zea, Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes , Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis , rapeseed, Carthamus, Glycine, Soja, Helianthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale , or other members of the Gramineae.

5. The method of claim 1 , wherein the plant material is a plant or a seed.

6. The method of claim 1 , wherein the nucleic acid molecule of claim 1 further comprises a gene regulatory element.

7. The method of claim 1 , wherein the nucleic acid molecule of claim 6 , wherein the gene regulatory element is the phaseolin promoter or the LTP170 promoter.

8. The method of claim 1 , wherein the Δ9 desaturase enzyme comprises a serine at the position analogous to position 114 in SEQ ID NO:2; an arginine at the position analogous to position 117 in SEQ ID NO:2; a cysteine at the position analogous to position 118 in SEQ ID NO:2, a leucine at the position analogous to position 179 in SEQ ID NO:2; or a threonine at the position analogous to position 188 in SEQ ID NO:2.

9. The method of claim 1 , wherein the Δ9 desaturase enzyme comprises a serine at the position analogous to position 114 in SEQ ID NO:2; an arginine at the position analogous to position 117 in SEQ ID NO:2; a cysteine at the position analogous to position 118 in SEQ ID NO:2, a leucine at the position analogous to position 179 in SEQ ID NO:2; and a threonine at the position analogous to position 188 in SEQ ID NO:2.

10. A plant material comprising each of:
two means for increasing levels of 16:0 ACP in the plant material;
an extraplastidial palmitic acid (16:0) desaturase;
a Δ9 desaturase enzyme that comprises an amino acid sequence at least 80% identical to SEQ ID NO:2; and
a polynucleotide is at least 60% identical to SEQ ID NO:1 that encodes the Δ9 desaturase enzyme.

11. The plant material of claim 10 , wherein the plant material is a plant.

12. The plant material of claim 10 , wherein the plant material is a seed.

13. The method according to claim 1 , wherein the extraplastidial palmitic acid (16:0) desaturase is a fungal desaturase.

14. The method according to claim 13 , wherein the extraplastidial palmitic acid (16:0) desaturase is selected from the group consisting of LnΔ9D desaturase and AnΔ9 desaturase.

15. The plant material of claim 10 , wherein the extraplastidial palmitic acid (16:0) desaturase is a fungal desaturase.

16. The plant material of claim 15 , wherein the extraplastidial palmitic acid (16:0) desaturase is selected from the group consisting of LnΔ9D desaturase and AnΔ9 desaturase.

Assignees

• Brookhaven Science Ass Llc
• Dow Agrosciences Llc

Inventors

• Shanklin John
• Nguyen Tam Huu
• Walsh Terence A
• Pidkowich Mark S
• Whittle Edward J

Classifications

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N9/0083

  Miscellaneous (1.14.99) · CPC title

• C12Y114/19001

  Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title