Compositions and methods for producing tobacco plants and products having altered alkaloid levels with desirable leaf quality
US-11859192-B2 · Jan 2, 2024 · US
US9974275B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9974275-B2 |
| Application number | US-201615008717-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 28, 2016 |
| Priority date | Jan 30, 2015 |
| Publication date | May 22, 2018 |
| Grant date | May 22, 2018 |
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The present invention relates to a Spinacia oleracea seed designated 51-341 RZ and 51-342 RZ, which exhibits a combination of traits including very late bolting, slow growing, a semi-erect plant habit, a small leaf blade size and resistance to downy mildew ( P. farinosa f. sp. spinaciae ) races Pfs1 to 15. The present invention also relates to a Spinacia oleracea plant produced by growing the 51-341 RZ or 51-342 RZ seed. The invention further relates to methods for producing the spinach cultivar, represented by spinach variety 51-341 RZ and 51-342 RZ.
Opening claim text (preview).
What is claimed is: 1. A seed of hybrid spinach variety 51-341 RZ or 51-342 RZ, a sample of seed of said hybrid variety having been deposited under NCIMB Accession Number 42353 and 42530, respectively. 2. A plant grown from either the seed of hybrid spinach variety 51-341 RZ or the seed of hybrid spinach variety 51-342 RZ of claim 1 . 3. The spinach plant of claim 2 , which is a plant grown from seed having been deposited under NCIMB Accession Number 42353 or 42530. 4. A spinach plant, or a part thereof, having all the physiological and morphological characteristics of the spinach plant of claim 2 . 5. A part of the plant of claim 2 , wherein said part is a microspore, pollen, an ovary, an ovule, an embryo sac, an egg cell, a cutting, a root, a stem, a cell or a protoplast. 6. A tissue culture of regenerable cells or protoplasts from the plant part of claim 5 . 7. The tissue culture as claimed in claim 6 , wherein said cells or protoplasts of the tissue culture are derived from a leaf, pollen, an embryo, a cotyledon, a hypocotyl, a meristematic cell, a root, a root tip, an anther, a flower, a seed or a stem. 8. A spinach plant regenerated from the tissue culture of claim 6 , wherein the regenerated plant expresses all of the physiological and morphological characteristics of hybrid spinach variety 51-341 RZ or 51-342 RZ, a sample of seed of said hybrid having been deposited under NCIMB Accession Number 42353 and 42530, respectively. 9. A method of vegetatively propagating a plant of hybrid spinach variety 51-341 RZ or 51-342 RZ comprising the steps of: (a) collecting tissue capable of being propagated from a plant of hybrid spinach variety 51-341 RZ or 51-342 RZ, representative seed of said hybrid spinach variety having been deposited under NCIMB Accession Number 42353 and 42530, respectively; and (b) producing a rooted plant from said tissue. 10. A method of producing a spinach seed, comprising crossing the plant of claim 2 with itself or a second spinach plant. 11. A method for producing a seed of a hybrid spinach variety 51-341 RZ or 51-342 RZ-derived spinach plant comprising the steps of: (a) crossing a spinach plant of hybrid spinach variety 51-341 RZ or 51-342 RZ, representative seed of which having been deposited under NCIMB Accession Number 42353 and 42530, respectively, with a second spinach plant or with itself; and (b) allowing seed of a 51-341 RZ or 51-342 RZ-derived spinach plant to form. 12. The method of claim 11 , further comprising the steps of: (c) selfing the plant grown from said 51-341 RZ or 51-342 RZ-derived spinach seed or crossing it to a second spinach plant to yield additional 51-341 RZ or 51-342 RZ-derived spinach seed; (d) growing said additional 51-341 RZ or 51-342 RZ-derived spinach seed of step (c) to yield additional 51-341 RZ or 51-342 RZ-derived spinach plants; and (e) repeating the crossing and growing steps of (c) and (d) for an additional 3-10 generations to generate further 51-341 RZ or 51-342 RZ-derived spinach plants, and (f) allowing seed of a further 51-341 RZ or 51-342 RZ-derived spinach plant to form. 13. A method of producing a plant of spinach hybrid variety 51-341 RZ or 51-342 RZ comprising at least one new trait, the method comprising introducing a mutation or transgene conferring the at least one new trait directly into a plant of hybrid spinach variety 51-341 RZ or 51-342 RZ, wherein a sample of seed of said variety has been deposited under NCIMB Accession Number 42353 and 42530, respectively. 14. The spinach plant produced by the method of claim 13 . 15. A method of producing spinach leaves comprising: (a) obtaining a plant according to claim 2 , wherein the plant has been cultivated to obtain leaves; and (b) harvesting spinach leaves from the plant to thereby obtain harvested spinach leaves. 16. The method of claim 15 further comprising packaging the harvested spinach leaves as a fresh vegetable. 17. The method of claim 15 further comprising processing the harvested spinach leaves as a processed food. 18. A container comprising one or more spinach plants of claim 2 for harvest of leaves. 19. A method of determining the genotype of a plant of hybrid spinach variety 51-341 RZ or 51-342 RZ, representative seed of which having been deposited under NCIMB Accession Number 42353 and 42530, respectively, or a first generation progeny thereof, comprising obtaining a sample of nucleic acids from said plant and comparing said nucleic acids to a sample of nucleic acids obtained from a reference plant, and detecting a plurality of polymorphisms between the two nucleic acid samples, wherein the plurality of polymorphisms are indicative of hybrid spinach variety 51-341 RZ or 51-342 RZ and/or give rise to the expression of any one or more, or all, of the morphological and physiological characteristics of hybrid spinach variety 51-341 RZ and/or 51-342 RZ as claimed in claim 2 . 20. A plant of spinach hybrid variety 51-341 RZ or 51-342 RZ comprising a transgene conferring a desired trait, a sample of seed of said variety having been deposited under NCIMB Accession Number 42353 and 42530, respectively. 21. A seed that produces the plant of claim 20 .
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