Processing biomass
US-2015353974-A1 · Dec 10, 2015 · US
US9970157B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9970157-B2 |
| Application number | US-201414910178-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 7, 2014 |
| Priority date | Aug 9, 2013 |
| Publication date | May 15, 2018 |
| Grant date | May 15, 2018 |
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The present invention provides an enzymatic method for reducing the content of hexenuronic acids in a chemical cellulosic pulp and/or improvement of the brightness of cellulosic pulp using haloperoxidase.
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The invention claimed is: 1. A method for reducing the content of hexenuronic acids in and/or improving the brightness of a cellulosic pulp, said method comprising: contacting said pulp with a haloperoxidase comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 1, hydrogen peroxide, and chloride, bromide, iodide, and/or thiocyanate ions. 2. The method of claim 1 , wherein said haloperoxidase comprises an amino acid sequence that is at least 97% identical to SEQ ID NO: 1. 3. The method of claim 1 , wherein said haloperoxidase comprises an amino acid sequence that is 100% identical to SEQ ID NO: 1. 4. The method of claim 1 , further comprising contacting said pulp with a xylanase. 5. The method of claim 1 , further comprising contacting said pulp with an endo-1,4-beta-xylanase from enzyme class EC 3.2.1.8. 6. The method of claim 1 , further comprising contacting said pulp with a xylanase comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 3. 7. The method of claim 1 , further comprising contacting said pulp with a xylanase comprising an amino acid sequence that is at least 97% identical to SEQ ID NO: 3. 8. The method of claim 1 , wherein said pulp is a pulp made by alkaline cooking. 9. An aqueous composition, comprising: a cellulosic pulp comprising one or more hexenuronic acids; a haloperoxidase comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 1; hydrogen peroxide; and chloride, bromide, iodide, or thiocyanate ions. 10. The composition of claim 9 , wherein said pulp is a pulp made by alkaline cooking. 11. The composition of claim 9 , further comprising a xylanase. 12. The method of claim 1 , further comprising contacting said pulp with a xylanase comprising an amino acid sequence is 100% identical to SEQ ID NO: 3. 13. The method of claim 1 , further comprising contacting said pulp with one or more tertiary amines. 14. A method for reducing the content of hexenuronic acids in and/or improving the brightness of a cellulosic pulp, said method comprising: contacting said pulp with a haloperoxidase comprising an amino acid sequence that is 100% identical to SEQ ID NO:1; a xylanase comprising an amino acid sequence that is 100% identical to SEQ ID NO: 3; hydrogen peroxide; and chloride, bromide, iodide, and/or thiocyanate ions. 15. The method of claim 14 , further comprising contacting said pulp with one or more tertiary amines. 16. The composition of claim 9 , wherein said haloperoxidase comprises an amino acid sequence that is at least 97% identical to SEQ ID NO: 1. 17. The composition of claim 9 , wherein said haloperoxidase comprises an amino acid sequence that is 100% identical to SEQ ID NO: 1. 18. The composition of claim 11 , wherein said xylanase comprises an amino acid sequence that is at least 95% identical to SEQ ID NO: 3. 19. The composition of claim 11 , wherein said xylanase comprises an amino acid sequence that is at least 97% identical to SEQ ID NO: 3. 20. The composition of claim 11 , wherein said xylanase comprises an amino acid sequence that is 100% identical to SEQ ID NO: 3.
with halogens or halogen-containing compounds (D21C9/16 takes precedence) · CPC title
with peroxides · CPC title
with ClO2/Cl2 and other bleaching agents in a multistage process · CPC title
organic compounds · CPC title
Treatment of cellulose-containing material with microorganisms or enzymes · CPC title
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