High-yield purification method for target protein
US-2024059730-A1 · Feb 22, 2024 · US
US9968868B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9968868-B2 |
| Application number | US-201514934239-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 6, 2015 |
| Priority date | Jul 9, 2015 |
| Publication date | May 15, 2018 |
| Grant date | May 15, 2018 |
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Provided is a pore water extraction kit for extracting pore water in a seabed sediment. More specifically, provided is a pore water extraction kit of a seabed sediment for more easily extracting pore water separated from a sediment through a centrifugal separator tube.
Opening claim text (preview).
What is claimed is: 1. A pore water extraction kit of a seabed sediment, comprising: a centrifugal separator tube including a sample accommodating space formed therein so as to store a sample, an extraction hole formed in an outer surface thereof in order to extract pore water separated from the sample, and an elastic membrane sealing the extraction hole; an extractor including an enclosed body that includes opposite facing sidewalls, wherein a pore water accommodating space provided between the sidewalls of the body so as to accommodate the pore water and a needle is attached on one sidewall so as to penetrate through the elastic membrane to allow the sample accommodating space and the pore water accommodating space to be in communication with each other, the extractor further including a needle for discharging, wherein the needle for discharging is provided on an opposite sidewall of the extractor from the needle to penetrate through the elastic membrane; and a cap, wherein the centrifugal separator tube has an opening in an upper side thereof and is openable and closable by the cap, and the cap is provided with a compressible button fitted to an inner diameter of the opening in the upper side of the centrifugal separator tube so as to apply pressure to the sample accommodating space for causing pore water to be communicated to the extractor. 2. The pore water extraction kit of a seabed sediment of claim 1 , wherein a plurality of extraction holes are disposed to be spaced apart from each other in a length direction of the centrifugal separator tube. 3. The pore water extraction kit of a seabed sediment of claim 1 , wherein the extractor includes a filter disposed at an introduction side of the pore water accommodating space and configured to filter the pore water introduced into the pore water accommodating space from the sample accommodating space. 4. A pore water extraction kit of a seabed sediment, comprising: a centrifugal separator tube having a sample accommodating space formed therein for storing a sample, an extraction hole formed in an outer surface thereof for extracting pore water separated from the sample, an elastic membrane sealing the extraction hole, and a cap, wherein the centrifugal separator tube has an open upper side for communicating the sample accommodating space with the exterior of the tube, the cap is movable to close the open upper side of the centrifugal separator tube and comprises a compressible button for applying pressure to the sample accommodating space, wherein the compressible button is fitted to an inner diameter of the open upper side of the centrifugal separator tube; and an extractor including an enclosed body that includes opposite facing sidewalls, wherein a pore water accommodating space is provided between the sidewalls of the body so as to accommodate the pore water and a needle is attached on one sidewall and configured to penetrate through the elastic membrane to allow the sample accommodating space and the pore water accommodating space to be in communication with each other. 5. The pore water extraction kit of a seabed sediment of claim 4 , wherein the extractor further comprises: a needle for discharging provided on an opposite sidewall of the extractor from the needle. 6. The pore water extraction kit of a seabed sediment of claim 4 , wherein the centrifugal separator tube has a plurality of extraction holes formed therein and disposed to be spaced apart from each other in a length direction of the centrifugal separator tube. 7. The pore water extraction kit of a seabed sediment of claim 4 , wherein the extractor includes a filter disposed at an introduction side of the pore water accommodating space and filtering the pore water introduced into the pore water accommodating space.
filtration · CPC title
by using a centrifuge · CPC title
by dialysis, osmosis or reverse osmosis {(general membrane separation processes B01D61/00, membrane modules B01D63/00, electrodialysis C02F1/4693, combination of membrane modules and bioreactors C02F3/1268)} · CPC title
cylindrical, tube shaped · CPC title
Control of the sequence of chambers filled or emptied · CPC title
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