Methods for sampling gingival metabolites

Patent metadata

Field	Value
Publication number	US-9964472-B2
Application number	US-201615197505-A
Country	US
Kind code	B2
Filing date	Jun 29, 2016
Priority date	Jun 29, 2016
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

One or more methods for sampling gingival metabolites and biomarkers.

First claim

What is claimed is:

1. A method for non-invasive collection of gingival samples comprising:
using a collection device oriented parallel to the gum line of a specific tooth; from a subject collecting said gingival samples
detaching a portion of the collection device from the collection device and placing into a container;
extracting protein and metabolite from the portion of the collecting device.

2. The method of claim 1 wherein the collection device is at least one of an interdental gum brush or buccal brush.

3. The method of claim 1 wherein the container contains extraction buffer.

4. The method of claim 3 wherein the extraction buffer is at least one of Dulbecco's phosphate-buffered saline or RNAlater.

5. A method for extraction from gingival brush samples comprising:
using a collection device oriented parallel to the gum line of a specific tooth; from a subject collecting said gingival samples
a portion of the collection device is detached from the collection device and placed into a container containing extraction buffer;
removing the collection device from the container; and
extracting a biomarker.

6. The method of claim 5 , wherein the extracted biomarker is analyzed with analytical procedures that quantify the levels of at least one of metabolite.

7. The method of claim 6 , wherein a metabolite is at least one of a metabolite produced by: lipid metabolism, protein and amino acid metabolism, carbohydrate metabolism, nuclear acid metabolism, or oxidative phosphorylation.

8. The method of claim 6 , wherein the method of analysis is at least one of: immunoassay, gradient hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC/MS/MS), enzymatic assay; or colorimetric assay.

9. The method of claim 6 , wherein the metabolite is at least one of: citrulline, ornithine, arginine, succinate, malate and fumarate, deoxycarnitine or pyroglutamine.

10. The method of claim 6 , wherein the metabolite is at least one of: 13-HODE (13-hydroxyoctadecadienoic acid), 9-HODE, asparagylleucine, arginylphenylalanine, valylvaline, valine, tyrosine, tryptophan, arginylleucine, trehalose, threonylphenylalanine, threonylleucine, alanine, spermidine, 2-methylbutyrylcarnitine (C5), serylisoleucine, pyroglutamine, adenosine 5′-monophosphate, propionylcarnitine, proline, 1-oleoylglycerophosphoethanolamine, phosphate, palatinitol, mannitol, maltotriose, maltose, lysylphenylalanine, lysylleucine, leucylleucine, leucine, glycerol, lauryl sulfate, glutathione, oxidized glutathione, glutamine, fructose, erythritol, or 1-arachidonoylglycerophosphoethanolamine.

Assignees

• Procter & Gamble

Inventors

• Xie Sancai
• Haught John Christian
• Tansky Cheryl Sue
• Huggins Jr Thomas Glenn
• White Jr Donald James
• Li Lijuan
• Klukowska Malgorzata
• Fieno Angela Marie
• Khambe Deepa Ashok

Classifications

• G01N1/28 Primary

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title

• A61B10/02

  Instruments for taking cell samples or for biopsy {(A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48)} · CPC title

• A61B2010/0225

  for taking multiple samples · CPC title

• A61B2010/0216

  Sampling brushes · CPC title

• A61Q11/00 Primary

  Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses · CPC title