Polypeptide electrospun nanofibrils of defined composition

Patent metadata

Field	Value
Publication number	US-9963805-B2
Application number	US-201615243423-A
Country	US
Kind code	B2
Filing date	Aug 22, 2016
Priority date	Jun 21, 2010
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

Electrospun nanofibrils and methods of preparing the same are provided. The electrospun nanofibrils comprise at least one polypeptide. A polypeptide can be dissolved in a solution, and the solution can be electrospun into a nanofibril. The solution can be added to a syringe or syringe pump, and an electric field can be applied to electrospin the at least one polypeptide.

First claim

We claim:

1. A method of preparing an electrospun nanofibril, comprising:
dissolving at least one polypeptide in a solvent to form a solution; and
electrospinning the solution to form the electrospun nanofibril,
wherein the at least one polypeptide is a random copolymer,
wherein a first monomer of the random copolymer is ornithine, and
wherein a second monomer of the random copolymer is lysine, alanine, glycine, proline, valine, or serine.

2. The method according to claim 1 , wherein a diameter of the nanofibril is less than a micron.

3. The method according to claim 1 , wherein electrospinning the solution comprises electrospinning the solution in an electric field of from about 10 2 V/m to about 7×10 5 V/m.

4. The method according to claim 1 , wherein the solvent is water.

5. The method according to claim 1 , wherein the at least one polypeptide is present in the solution at a concentration in a range of from 10% to 55% (w/v).

6. The method according to claim 1 , wherein the at least one polypeptide is present in the solution at a concentration in a range of from about 50% to about 55% (w/v).

7. The method according to claim 5 , wherein the at least one polypeptide is dissolved in the solvent at a temperature in a range of from 20° C. to 30° C.

8. The method according to claim 7 , wherein the solvent is water.

9. The method according to claim 1 , wherein the at least one polypeptide satisfies the opposite charges criterion.

10. The method according to claim 1 , wherein electrospinning the solution comprises continuously electrospinning the solution at a flow rate in a range of from 1 μL/min to 100 μL/min.

11. The method according to claim 8 , wherein electrospinning the solution comprises continuously electrospinning the solution at a flow rate in a range of from 1 μL/min to 100 μL/min.

12. The method according to claim 11 , wherein the at least one polypeptide satisfies the following equation: (non-polar surface area−polar surface area)/DP<100 square angstroms/residue, wherein DP is the degree of polymerization of the at least one polypeptide, wherein the at least one polypeptide satisfies the opposite charges criterion, and wherein the degree of polymerization of the at least one polypeptide is at least 25.

13. The method according to claim 1 , wherein the degree of polymerization of the at least one polypeptide is at least 25.

14. The method according to claim 1 , wherein the at least one polypeptide satisfies the following equation: (non-polar surface area−polar surface area)/DP<100 square angstroms/residue, wherein DP is the degree of polymerization of the at least one polypeptide.

Assignees

• Univ South Florida
• Univ Central Florida Res Found Inc

Inventors

• Haynie Donald T
• Zhai Lei

Classifications

• Y10T428/298

  Physical dimension · CPC title

• C08G69/10

  Alpha-amino-carboxylic acids {(polysuccinimides C08G73/1092)} · CPC title

• D01F6/68 Primary

  from polyaminoacids or polypeptides · CPC title

• D01D5/0007

  Electro-spinning (non-woven fabrics produced by electro-spinning D04H1/728) · CPC title

• D01D5/003

  the material being a polymer solution or dispersion (D01D5/0053 takes precedence) · CPC title