Thermochromic sensing devices, systems, and methods

Patent metadata

Field	Value
Publication number	US-9963732-B2
Application number	US-201514984739-A
Country	US
Kind code	B2
Filing date	Dec 30, 2015
Priority date	Dec 30, 2015
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

One or more live substances is cultured at a plurality of test locations of a test vessel. The test locations include a thermochromic material and one or more test substances. A spectral shift in light emanating from the thermochromic material of the test locations is detected. The spectral shift occurs in response to an increase or decrease in energy conversion by the live substance. An effect of the one or more test substances on the live substances is determined based on the detected spectral shift.

First claim

The invention claimed is:

1. A system, comprising:
1) an incubation chamber including a compartment configured to receive a test vessel having one or more test substances, one or more live substances, and one or more thermochromic materials that react in response to heat generated by the live substances disposed at one or more test locations;
2) a measurement light source comprising one or more light emitters configured to emit measurement light; and
3) a detector subsystem comprising one or more photosensing elements configured to sense light emanating from the thermochromic material in response to the measurement light, the detector subsystem configured to provide an electrical signal that includes information about a spectrum of the light emanating from the thermochromic material, the spectrum of the emanating light indicative of a metabolism of the one or more live substances that causes a change in temperature of the thermochromic materials.

2. The system of claim 1 , wherein the test vessel comprises a standard test plate and the one or more locations comprises test wells of the test plate.

3. The system of claim 1 , further comprising an analyzer configured to determine a spectral shift of the light emanating from the thermochromic material at each test location based on the electrical signal.

4. The system of claim 3 , wherein the analyzer is further configured to determine an effect of one or more test substances on one or more live substances based on the spectral shift, the test substances and the live substances disposed at the test locations.

5. The system of claim 4 , wherein the analyzer is configured to determine a minimum inhibitory concentration of the test substances with respect to the live substances.

6. The system of claim 4 , further comprising a display, wherein the analyzer is configured to update the display with effects of multiple test substances on the one or more live substances and/or to generate an alert based on the effects of the one or more test substances.

7. The system of claim 1 , wherein the measurement light is optically multiplexed to the test locations and/or the light emanating from the test locations is demultiplexed to the one or more photosensing elements.

Assignees

• Palo Alto Res Ct Inc

Inventors

• Martini Joerg
• Recht Michael I
• Kiesel Peter

Classifications

• C12Q1/10

  Enterobacteria · CPC title

• C12Q1/18

  Testing for antimicrobial activity of a material · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title

• C12Q1/025 Primary

  for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics (antimicrobial activity C12Q1/18) · CPC title

• G01K11/12

  using changes in colour, translucency or reflectance · CPC title