Strain secreting fatty acids by phospholipase and method for producing fatty acids using it

Patent metadata

Field	Value
Publication number	US-9963722-B2
Application number	US-201514730174-A
Country	US
Kind code	B2
Filing date	Jun 3, 2015
Priority date	Jun 3, 2014
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

A bacterial strain secreting fatty acids, the strain inducing fatty acids to be extracellularly secreted by using phospholipase expressed in the periplasmic space of cell. When a method of producing fatty acids by using the bacterial strain secreting fatty acids is used, fatty acids extracellularly secreted are continuously obtained without apoptosis, leading to lower costs and higher production efficiency. Phospholipase, unlike thioesterase, which is a typical fatty-acid degrading enzyme, decomposes phospholipid to produce free fatty acids. Accordingly, by using the substrate specificity of two different phospholipases, a fatty acid having a specific composition can be selectively produced. Unlike in a typical method in which fat is obtained from cells or tissues, fatty acids secreted during cell growth are obtainable by biding to a hydrophobic material without an extraction process using an organic solvent in large quantities. Accordingly, a more economical, environmentally friendly bio-oil production process can be realized.

First claim

What is claimed is:

1. A bacterial strain, which comprises a gene coding cytochrome c2 signal peptide ligated to a gene coding phospholipase, for secreting fatty acids toward the outside a cell by expressing the phospholipase in the periplasmic space of a bacterial cell, wherein the bacterial strain is DH5α(pRK-fabD+pIND4-cycApLA1) deposited under the Access number of KCTC12599BP or DH5α(pRK-fabH+pIND4-cycApLA2) deposited under the Access number of KCTC12600BP.

2. A method of producing fatty acids, the method comprising culturing the strain of claim 1 in medium.

3. The method of claim 2 , wherein the medium comprises a carbon source, wherein the carbon source is selected from the group consisting of glucose and glycerol.

4. The method of claim 2 , wherein the carbon source in medium has an amount of 0.1% (w/v) to 4.0% (w/v).

Assignees

• Univ Sogang Res Foundation

Inventors

• Lee Jeong Kug
• Kim Eui Jin
• Tong Xiaomeng
• Oh Eun Kyung

Classifications

• C12N9/18

  Carboxylic ester hydrolases {(3.1.1)} · CPC title

• C12N9/93

  Ligases (6) · CPC title

• C12Y301/01004

  Phospholipase A2 (3.1.1.4) · CPC title

• C12Y203/0118

  Beta-ketoacyl-acyl-carrier-protein synthase III (2.3.1.180) · CPC title

• C12Y602/01003

  Long-chain-fatty-acid-CoA ligase (6.2.1.3) · CPC title