Cell washing device using a wave

Patent metadata

Field	Value
Publication number	US-9963693-B2
Application number	US-201414519302-A
Country	US
Kind code	B2
Filing date	Oct 21, 2014
Priority date	Oct 21, 2013
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed.

First claim

What is claimed is:

1. A method of separating a component from a wash material, the method comprising:
pumping a sample and the wash material into a separating system with a substantially steady flow rate, wherein pumping is established and maintained by at least one rotor pump and a baffle, the baffle comprising an inlet, an outlet, and an expansion chamber located in between the inlet and the outlet, wherein pumping the sample and the wash material into to the separating system includes pumping the sample and the wash material into the baffle prior to the sample and the wash material entering the separating system such that air is trapped in the expansion chamber to absorb fluid pulses generated by the rotor pump;
separating the component from the wash material;
collecting the component.

2. The method according to claim 1 , further comprising mixing the sample with the wash material to form a mixture and pumping the mixture into the baffle and the separation system, wherein the at least one rotor pump is a single rotor pump.

3. The method according to claim 1 , wherein pumping comprises pumping the sample into the baffle and the separation system with a first pump of the at least one rotor pump and pumping the wash material into the baffle and the separation system with a second pump of the at least one rotor pump, wherein the sample and the wash material mix within the separation system.

4. The method according to claim 1 , wherein pumping comprising pumping the sample and the wash solution at a flow rate of about 1.0 to about 1.5 mils with a variance of about 50 to about 130 Ws.

5. The method according to claim 1 , wherein separating the component from the wash material comprises flowing the component and the wash material through a flow channel positioned relative to a first wave generator and a second wave generator, wherein the first and second wave generators generate a standing surface acoustic wave, and wherein the standing surface acoustic wave focuses the component to a component collection path separate from a wash material collection path.

6. A method of separating a component from a wash material, the method comprising:
obtaining a separation system comprising a flow channel having a mixing section, a focused flow region, a first exit port, a first wave generator, and a second wave generator, wherein internal surfaces of the flow channel, the mixing section, and the focused flow region are coated with a hydrophilic material;
pumping a sample comprising a component into a flow channel of a separation system;
pumping the wash material into the flow channel of the separation system;
generating a standing surface acoustic wave relative to the focused flow region of the flow channel using the first wave generator and the second wave generator; and
separating the component from the wash material by directing the sample through the focused flow region to expose the component and the wash material to the standing surface acoustic wave.

7. The method according to claim 6 , wherein pumping the sample comprising the component into the flow channel and pumping the wash material into the flow channel comprises pumping the sample and the wash material into the mixing section of the flow channel, wherein the sample and the wash material are mixed together.

8. The method according to claim 6 , wherein pumping the sample comprising the component is performed with a first pump and pumping the wash material is performed with a second pump.

9. The method according to claim 6 , wherein separating comprises:
flowing the sample comprising the component and the wash material through the flow channel, wherein the standing surface acoustic wave moves the component to the focused flow region of the flow channel; and
collecting the component at the first exit port.

10. The method according to claim 9 , wherein the flow channel has a second exit port and the method further comprises collecting the wash material at the second exit port.

11. The method according to claim 6 , wherein at least one of pumping the sample or pumping the wash material comprises pumping with a rotor pump that establishes a substantially steady flow rate.

12. The method according to claim 6 , wherein the component comprises red blood cells and the wash material is selected from a group consisting of water, saline, dextrose, and mixtures thereof, wherein separating the component from the wash material comprises separating the red blood cells from the wash material.

13. The method according to claim 6 , further comprising rejuvenating a sample of red blood cells with a rejuvenation solution to generate rejuvenated red blood cells, the component including the rejuvenated red blood cells.

14. The method according to claim 13 , wherein separating the component from the wash material further comprises separating the rejuvenated red blood cells from the rejuvenation solution.

15. A method of separating red blood cells from a wash material, the method comprising:
transferring the red blood cells and the wash material into a mixing chamber;
mixing the red blood cells with the wash material within the mixing chamber to form a mixture;
pumping the mixture from the mixing chamber to a separation system comprising a flow channel; a first wave generator, and a second wave generator, wherein the first and second wave generators generate a standing surface acoustic wave relative to the flow channel;
separating the red blood cells from the wash material by flowing the mixture through the flow channel relative the standing surface acoustic wave, wherein separating the red blood cells from the wash material further includes injecting a directing flow into the flow channel to direct the red blood cells towards a red blood cell collection path; and
collecting the red blood cells from the red blood cell collection path, the red blood cell collection path having a smaller exit size than an exit size of a wash material collection path.

16. The method of claim 15 , further comprising rejuvenating the red blood cells by mixing the red blood cells with a rejuvenating solution, wherein separating further comprises separating the red blood cells from the rejuvenating solution.

17. The method according to claim 15 , wherein mixing comprises mixing the red blood cells and the wash material at about 30° C. to about 36° C. for about 2 minutes to about 10 minutes.

18. The method according to claim 15 , further comprising infusing the red blood cells to a patient.

Assignees

• Biomet Biologics Llc

Inventors

• Hamman Ned M
• Leach Michael D
• Abeskarón David
• Cunningham Grant D

Classifications

• B01D21/283 Primary

  Settling tanks provided with vibrators · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• A61M1/0209

  Multiple bag systems for separating or storing blood components · CPC title

• G01N2015/1006

  for cytology · CPC title

• A61K2035/124

  the cells being hematopoietic, bone marrow derived or blood cells · CPC title