Methods and compositions for synthesizing improved silk fibers

Patent metadata

Field	Value
Publication number	US-9963554-B2
Application number	US-201615073514-A
Country	US
Kind code	B2
Filing date	Mar 17, 2016
Priority date	Sep 17, 2013
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

First claim

What is claimed is:

1. A proteinaceous block co-polymer, comprising:
a polypeptide, the polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof.

2. The proteinaceous block co-polymer of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40%, a glycine composition from 25 to 50%, a proline composition from 9 to 20%, a β-turn composition from 15 to 37%, a GPG amino acid motif content from 18 to 55%, and a poly-alanine amino acid motif content from 9 to 35%.

3. The proteinaceous block co-polymer of claim 1 , consisting of: 3 concatenated repeats of SEQ ID NO: 1396.

4. A fiber comprising the block co-polymer of claim 1 .

5. The fiber of claim 4 , comprising a circular cross-section and a diameter from 4.48 to 12.7 μm.

6. The fiber of claim 4 , wherein the fiber has a yield stress from 24 to 172 MPa.

7. The fiber of claim 4 , wherein the fiber has a maximum stress from 54 to 310 MPa.

8. The fiber of claim 4 , wherein the fiber has a breaking strain from 2 to 200%.

9. The fiber of claim 4 , wherein the fiber has an initial modulus from 1617 to 5820 MPa.

10. The fiber of claim 4 , wherein the fiber has a toughness value from 0.5 MJ/m 3 to 59.2 MJ/m 3 .

11. A culture medium comprising a secreted block copolymer of claim 1 .

Assignees

• Bolt Threads Inc

Inventors

• Widmaier Daniel M
• Breslauer David N
• Kittleson Joshua
• Turner Brendan
• Wray Lindsay

Classifications

• D01F4/02

  from fibroin · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• D01D5/00

  Formation of filaments, threads, or the like · CPC title

• C07K2319/02

  containing a signal sequence · CPC title

• B29C48/919

  using a bath, e.g. extruding into an open bath to coagulate or cool the material · CPC title