Antibody that specifically binds partially processed or unprocessed neurotoxin polypeptides

Patent metadata

Field	Value
Publication number	US-9963502-B2
Application number	US-201615230928-A
Country	US
Kind code	B2
Filing date	Aug 8, 2016
Priority date	Feb 19, 2009
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

The present invention relates to an antibody which specifically binds to unprocessed and/or partially processed neurotoxin polypeptide or an antibody which specifically binds an epitope consisting of a peptide having an amino acid sequence as shown in any one of SEQ ID NOs: 1 to 16 and to methods for the manufacture of such antibodies. Moreover, the present invention relates to a composition comprising processed neurotoxin polypeptide free of unprocessed or partially processed neurotoxin polypeptide and a method for manufacturing said neurotoxin polypeptide based on the antibodies of the invention. The present invention also relates to the use of the aforementioned antibody for separating processed neurotoxin polypeptides from unprocessed or partially processed neurotoxin polypeptides or for determining unprocessed or partially processed neurotoxin polypeptides. The present invention relates to a method for the manufacture of a medicament.

First claim

The invention claimed is:

1. An antibody coupled to a solid support, which antibody specifically binds an epitope, wherein the epitope is a peptide consisting of the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15 or SEQ ID NO: 16, and wherein the antibody specifically binds partially processed or unprocessed Clostridium neurotoxin polypeptides and does not cross react with completely processed Clostridium neurotoxin polypeptides.

2. The antibody of claim 1 , which is a monoclonal or polyclonal antibody.

Assignees

• Merz Pharma Gmbh & Co Kgaa

Inventors

• Pfeil Michael
• Friedrich Josef
• Taylor Harold Victor
• Eisele Karl-Heinz
• Brünn Cornelia

Classifications

• A61P27/02

  Ophthalmic agents · CPC title

• A61P25/08

  Antiepileptics; Anticonvulsants · CPC title

• A61P25/02

  for peripheral neuropathies · CPC title

• C07K16/1282 Primary

  Clostridium (G) · CPC title

• C07K5/0815

  with the first amino acid being basic · CPC title