Multiwell plate

Patent metadata

Field	Value
Publication number	US-9962694-B2
Application number	US-201715613004-A
Country	US
Kind code	B2
Filing date	Jun 2, 2017
Priority date	Jun 5, 2012
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

An assembly for processing a sample is provided. The assembly comprises a first body having a plurality of spaced-apart conduits and a second body having a plurality of chambers wherein each conduit is fluidically connected to a separate chamber. The assembly forms a plurality of liquid flow paths, each flow path comprising a conduit and a chamber. An analyte capture element is detachably attached to a conduit and is in fluidic communication with the liquid flow path of the conduit. Optionally, the assembly further may comprise a third body comprising a plurality of reservoirs. The assembly can be used to process a liquid sample for detecting an analyte.

First claim

The invention claimed is:

1. An assembly for processing a sample, comprising:
a first body comprising a plurality of spaced-apart conduits, the conduits configured in a linear array;
wherein the plurality of conduits comprises:
a first conduit having a first opening and a second opening;
a second conduit adjacent the first conduit, the second conduit having a first opening and a second opening;

a second body operably coupled thereto;
wherein the second body comprises a plurality of spaced-apart chambers; each chamber having conduit-receiving opening, an interior volume, and a drain;
wherein the plurality of spaced-apart chambers comprises:
a first chamber having a first interior volume and a first drain;
a second chamber adjacent the first chamber, the second chamber having a second interior volume and a second drain;

wherein, when the first body and the second body are operably coupled, a portion of the first conduit is disposed in the first interior volume forming a first flow path including the first drain, and a portion of the second conduit is disposed in the second interior volume forming a second flow path including the second drain; and

a capture element detachably attached to the first conduit and disposed in fluidic communication with the first flow path;
wherein a first shortest distance between the second opening of the first conduit and the second opening of the second conduit is shorter than a second shortest distance between the first drain and the second drain; and
further comprising a third body operably coupled to the first body of the assembly, wherein the third body comprises a plurality reservoirs, each reservoir comprising an outlet and the plurality of outlets forming a linear array, wherein the plurality of outlets comprises a first outlet and a second outlet wherein, when operably coupled to the first body of the assembly, the first outlet is placed in fluidic communication with the first conduit and the second outlet is placed in fluidic communication with the second conduit; and
wherein each of the outlets separately extends from the third body and is inserted at least partially into the corresponding conduit in the first body.

2. The assembly of claim 1 , wherein each of the plurality of chambers comprises a floor, wherein the floor of each of the plurality of chambers comprises the drain.

3. The assembly of claim 1 ;
wherein the capture element comprises a capture element depth;
wherein the assembly has a third shortest distance between the second opening of a conduit and the floor of the receiving chamber in which the portion of the conduit is disposed;
wherein in the capture element depth is longer than the third shortest distance.

4. An assembly for processing a sample, comprising:
a first body comprising a plurality of spaced-apart conduits, the conduits configured in a linear array;
wherein the plurality of conduits comprises:
a first conduit having a first opening and a second opening;
a second conduit adjacent the first conduit, the second conduit having a first opening and a second opening;

a second body operably coupled thereto;
wherein the second body comprises a plurality of spaced-apart chambers; each chamber having conduit-receiving opening, an interior volume, and a floor comprising a drain;
wherein the plurality of spaced-apart chambers comprises:
a first chamber having a first interior volume and s first floor comprising a first drain;
a second chamber adjacent the first chamber, the second chamber having a second interior volume and a second floor comprising a second drain;

wherein, when the first body and the second body are operably coupled, a portion of the first conduit is disposed in the first interior volume forming a first flow path including the first drain, and a portion of the second conduit is disposed in the second interior volume forming a second flow path including the second drain; and

a capture element detachably attached to the first conduit and disposed in fluidic communication with the first flow path, the capture element comprising a capture element depth;
wherein the assembly has a third shortest distance between the second opening of a conduit and the floor of the receiving chamber in which the portion of the conduit is disposed;
wherein in the capture element depth is longer than the third shortest distance; and
further comprising a third body operably coupled to the first body of the assembly, wherein the third body comprises a plurality reservoirs, each reservoir comprising an outlet and the plurality of outlets forming a linear array, wherein the plurality of outlets comprises a first outlet and a second outlet wherein, when operably coupled to the first body of the assembly, the first outlet is placed in fluidic communication with the first conduit and the second outlet is placed in fluidic communication with the second conduit; and
wherein each of the outlets separately extends from the third body and is inserted at least partially into the corresponding conduit in the first body.

5. The assembly of claim 4 , wherein a first shortest distance between the second opening of the first conduit and the second opening of the second conduit is shorter than a second shortest distance between the first drain and the second drain.

6. The assembly of claim 2 , wherein the floor further comprises a trough extending along a portion of the floor to the drain.

7. The assembly of claim 1 , wherein at least a portion of the capture element is disposed in the first conduit.

8. The assembly of claim 1 , further comprising a third body operably coupled to the first body of the assembly, wherein the third body comprises a plurality reservoirs, each reservoir comprising an outlet and the plurality of outlets forming a linear array, wherein the plurality of outlets comprises a first outlet and a second outlet wherein, when operably coupled to the first body of the assembly, the first outlet is placed in fluidic communication with the first conduit and the second outlet is placed in fluidic communication with the second conduit.

9. The assembly of claim 8 , wherein the first body or third body comprises a first positioning element configured to orient the third body in a predefined location with respect to the first body.

10. The assembly of claim 9 , wherein the first or third body further comprises a second positioning element configured to act cooperatively with the first positioning element to orient the third body in a predefined location with respect to the first body.

11. The assembly of claim 1 , wherein the first body is adapted to sealingly couple to the second body and/or the second body is adapted to sealingly couple to the first body.

12. The assembly of claim 1 , wherein the two or more outlets of the first body are shaped, dimensioned, and spaced apart such that each outlet individually can be received by a container.

13. The assembly of claim 1 , wherein the plurality of outlet openings is configured in a substantially linear arrangement, wherein at least three of the plurality of drains is configured in a saw-toothed arrangement.

14. The assembly of claim 1 , wherein the second body is adapted to be operationally connected to a vacuum source.

15. A kit, comprising:
a first body comprising a plurality of spaced-apart conduits, the conduits configured in a linear array;
wherein the plurality of conduits comprises:
a first conduit having a first opening and a second opening;
a second conduit adjacent the first conduit, the second conduit having a first opening and a second opening; and

a second body c

Assignees

• 3M Innovative Properties Co

Inventors

• Kirschhoffer Jon A
• Tilstra Andrew H
• Xia Wensheng

Classifications

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L2300/0851

  Bottom walls · CPC title

• B01L3/50255 Primary

  Multi-well filtration · CPC title

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title

• B01L2300/0681

  Filter · CPC title