Processing microtitre plates for covalent immobilization chemistries

US9962676B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9962676-B2
Application numberUS-201514674183-A
CountryUS
Kind codeB2
Filing dateMar 31, 2015
Priority dateNov 17, 2009
Publication dateMay 8, 2018
Grant dateMay 8, 2018

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  1. Title

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  2. Abstract

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  3. Assignees and inventors

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  4. Key dates

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  5. First independent claim

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  6. CPC / IPC classifications

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  7. Citations and related patents

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Abstract

Official abstract text for this publication.

Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

First claim

Opening claim text (preview).

What is claimed is: 1. A method comprising: treating an organic polymer microtitre plate with an electron beam-generated plasma; exposing the treated plate to air or an oxygen- and hydrogen-containing gas; wherein the treating and exposing generates hydroxyl groups on the surface of the plate. 2. The method of claim 1 ; wherein the plasma is generated in argon; wherein the argon gas pressure is 90 mTorr+/10%; wherein the treatment time is 2 min+/10%; wherein the electron beam is pulsed at 50 Hz+/−10%; and wherein the electron beam is operated with pulse width of 2 ms+/−10%.

Assignees

Inventors

Classifications

  • Treatment by wave energy or particle radiation (C08J7/18 takes precedence) · CPC title

  • B29C59/14Primary

    by plasma treatment {(plasma tubes per se H01J)} · CPC title

  • Light-directed synthesis · CPC title

  • with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

  • Polystyrene · CPC title

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Frequently asked questions

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What does patent US9962676B2 cover?
Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the s…
Who is the assignee on this patent?
North Stella H, Lock Evgeniya H, Walton Scott G, and 2 more
What technology area does this patent fall under?
Primary CPC classification B29C59/14. Mapped technology areas include Operations & Transport.
When was this patent published?
Publication date Tue May 08 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).