Sensors incorporating antibodies and methods of making and using the same

Patent metadata

Field	Value
Publication number	US-9958442-B2
Application number	US-201013201181-A
Country	US
Kind code	B2
Filing date	Feb 11, 2010
Priority date	Feb 11, 2009
Publication date	May 1, 2018
Grant date	May 1, 2018

Abstract

A sensor comprising an electronic circuit electrically coupled to a type III-V semiconductor material, for example indium arsenide (InAs) and an antibody contacting the type III-V semiconductor material. The sensor produces measurable N changes in the electrical properties of the semiconductor upon antibody-antigen binding events. Electrical properties measurable by the electronic device may include resistivity, capacitance, impedance, and inductance. A method of detecting an antigen using sensors of the invention. A method of detecting a reaction of an analyte to a stimulus using sensors of the invention. Sensor arrays comprising multiple sensors of the invention.

First claim

The invention claimed is:

1. A sensor comprising:
a type III-V semiconductor material having a first edge and a second edge,
an antibody contacting the type III-V semiconductor material, and
an electronic circuit electrically coupled to the type III-V semiconductor material,
the first edge of the type III-V semiconductor material configured for applying current to said first edge and the second edge configured for measuring voltage at said second edge to determine a first value of an electrical property of the semiconductor material, wherein the electronic circuit measures the electrical properly of the type III-V semiconductor material,
such that when an antigen binds to the antibody, the electrical property has a second value different from the first value.

2. The sensor of claim 1 , wherein the antibody comprises at least one of an IgG protein, an IgA protein, an IgM protein, an IgD protein, and an IgE protein.

3. The sensor of claim 1 , wherein the antibody binds an antigen selected from the group consisting of Tumor Necrosis Factor (TNF), Human Serum Albumin (HSA), Interleukin 1β (IL 1β), Interleukin 6 (IL 6), Follicle Stimulating Hormone (FSH), Interferon γ, or Human Chorionic Gonadotropin (HCG), and combinations thereof.

4. The sensor of claim 3 , wherein the antibody comprises at least one of an IgG protein, an IgA protein, an IgM protein, an IgD protein, and an IgE protein.

5. The sensor of claim 1 , wherein the antigen is selected from a prion, a protein, an amino acid, a nucleic acid, a carbohydrate, a hormone, a chemical compound, a chemical reaction intermediate, or a combination thereof.

6. The sensor of claim 5 , wherein the antibody comprises at least one of an IgG protein, an IgA protein, an IgM protein, an IgD protein, and an IgE protein.

7. The sensor of claim 1 having a van der Pauw configuration.

8. The sensor of claim 1 , further comprising a reservoir positioned to receive an analyte therein and contact the analyte with the type III-V material.

9. The sensor of claim 1 , wherein the electrical property is resistivity.

10. The sensor of claim 9 , wherein resistivity is sheet resistivity.

11. The sensor of claim 1 , wherein the type III-V semiconductor material comprises at least one of indium arsenide (InAs), gallium arsenide (GaAs), gallium nitride (GaN), indium nitride (InN) and a combination thereof.

12. The sensor of claim 1 , further comprising an additional antibody, the antibody binding a first antigen, the additional antibody binding a second antigen.

13. The sensor of claim 1 , further comprising a well.

14. A sensor array comprising a plurality of sensors of claim 1 .

15. The sensor array of claim 14 , comprising greater than ten sensors.

16. The sensor array of claim 14 , comprising at least two different antibodies.

Assignees

• Angelo R Michael
• Brown April S
• Wolter Scott
• Lampert William V
• Univ Duke

Inventors

• Angelo R Michael
• Brown April S
• Wolter Scott
• Lampert William V

Classifications

• G01N33/5438 Primary

  Electrodes · CPC title

• Y10T29/41

  Cross-Sectional Technologies · mapped topic