Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Patent metadata

Field	Value
Publication number	US-9957528-B2
Application number	US-201314440658-A
Country	US
Kind code	B2
Filing date	Nov 7, 2013
Priority date	Nov 9, 2012
Publication date	May 1, 2018
Grant date	May 1, 2018

Abstract

The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps:
a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product;

wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

First claim

The invention claimed is:

1. A process for preparation of a sugar product from ligno-cellulosic material, comprising:
enzymatic hydrolysis, in a reactor, of ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61;
wherein:
the enzymatic hydrolysis occurs for a first part of time and a subsequent second part of time; and
(i) during the first part of time oxygen is added to the ligno-cellulosic material to maintain a concentration of dissolved oxygen in the range of 0.02 mol/m 3 to 0.12 mol/m 3 , during the second part of time the concentration of dissolved oxygen is at a concentration at least 50% less than during the first part of time, and the first part of time is 2% to 80% of the total enzymatic hydrolysis time; or
(ii) during the first part of time dissolved oxygen in the ligno-cellulosic material is at a concentration that is at least 50% less than during the second part of time, during the second part of time oxygen is added to the ligno-cellulosic material to maintain a concentration of dissolved oxygen in the range of 0.02 mol/m 3 to 0.12 mol/m 3 , and the second part of time is 2% to 80% of the total enzymatic hydrolysis time;
and
dissolved oxygen is measured under normal atmospheric pressure and at about 62° C.;
the reactor has a volume of 1 m 3 or more; and
the ligno-cellulosic material has a dry matter content of 10 wt % or more.

2. The process according to claim 1 , wherein oxygen is added during the first part of time, and the first part of time is 2% to 30% of the total enzymatic hydrolysis time.

3. The process according to claim 2 , wherein the first part of time is 5% to 20% of the total enzymatic hydrolysis time.

4. The process according to claim 1 , wherein oxygen is added during the second part of time, and the second part of time is 12% to 50% of the total enzymatic hydrolysis time.

5. The process according to claim 4 , wherein the second part of time is 20% to 40% of the total enzymatic hydrolysis time.

6. The process according to claim 1 , wherein oxygen is added during the first part of time, and the dissolved oxygen concentration is maintained in the range of 0.02 mol/m 3 to 0.045 mol/m 3 .

7. The process according to claim 1 , wherein oxygen is added during the second part of time, and the dissolved oxygen concentration is maintained in the range of 0.02 mol/m 3 to 0.045 mol/m 3 .

8. The process according to claim 1 , wherein the total enzymatic hydrolysis time is 5 to 150 hours.

9. The process according to claim 1 , wherein the enzyme composition used retains activity for 30 hours or more.

10. The process according to claim 1 , wherein the hydrolysis is conducted at a temperature of 50° C. or more.

11. The process according to claim 1 , wherein the enzyme composition is from a fungus or the enzyme composition comprises a fungal enzyme.

12. The process according to claim 1 , wherein the dry matter content is 14 to 33 wt %.

13. The process according to claim 1 in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.

14. The process according to claim 1 in which oxygen is introduced as an oxygen-containing gas, in the form of bubbles.

15. The process according to claim 11 , wherein the enzyme composition is from a fungus, and the fungus is a microorganism of the genus Rasamsonia.

16. The process according to claim 11 , wherein the enzyme composition comprises a fungal enzyme, and the fungal enzyme is a Rasamsonia enzyme.

17. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material, prior to the enzymatic hydrolysis.

18. The process of claim 1 , further comprising washing of the ligno-cellulosic material, prior to the enzymatic hydrolysis.

19. The process of claim 1 , further comprising recovery of a sugar product from the enzymatic hydrolysis.

20. The process of claim 1 , further comprising, prior to the enzymatic hydrolysis, pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material.

21. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material prior to the enzymatic hydrolysis and recovery of a sugar product from the enzymatic hydrolysis.

22. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material, prior to the enzymatic hydrolysis, and recovery of a sugar product from the enzymatic hydrolysis.

23. The process of claim 1 , wherein oxygen is added during the first part of time, and during the second part of time the concentration of dissolved oxygen is at a concentration at least 70% less than during the first part of time.

24. The process according to of claim 1 , wherein oxygen is added during the first part of time, and during the second part of time the concentration of dissolved oxygen is at a concentration at least 90% less than during the first part of time.

25. The process of claim 1 , wherein oxygen is added during the second part of time, and during the first part of time the concentration of dissolved oxygen is at a concentration at least 70% less than during the second part of time.

26. The process of claim 1 , wherein oxygen is added during the second part of time, and during the first part of time the concentration of dissolved oxygen is at a concentration at least 90% less than during the second part of time.

27. The process of claim 1 , further comprising, following said enzymatic hydrolysis, fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product.

Assignees

• Dsm Ip Assets Bv

Inventors

• Noordam Bertus
• Berkhout Michael Petrus Jozef
• Hiseni Aida

Classifications

• C12P7/48

  Tricarboxylic acids, e.g. citric acid · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title

• C12P35/00

  Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin · CPC title

• C12P7/16

  Butanols · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title