Fungal resistant plants expressing CASAR

Patent metadata

Field	Value
Publication number	US-9957522-B2
Application number	US-201314074138-A
Country	US
Kind code	B2
Filing date	Nov 7, 2013
Priority date	Nov 13, 2012
Publication date	May 1, 2018
Grant date	May 1, 2018

Abstract

The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacopsoraceae in plants and/or plant cells. This is achieved by increasing the expression of a CASAR protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding a CASAR protein.

First claim

The invention claimed is:

1. A method for increasing fungal resistance in a plant, a plant part, or a plant cell, said method comprising transforming a plant, plant part, or plant cell with an exogenous nucleic acid encoding a Capsicum annuum systemic acquired resistance (CASAR) protein with at least 95% identity to SEQ ID NO:3,
wherein the CASAR protein confers increased resistance against rust thereto in comparison to a wild type plant, wild type plant part or wild type plant cell, and wherein the exogenous nucleic acid is in functional linkage with a heterologous rust-induced promoter.

2. The method of claim 1 , said method comprising:
(a) stably transforming a plant cell with an expression cassette comprising
an exogenous nucleic acid encoding a CASAR protein having at least 95% identity to SEQ ID NO: 3 in functional linkage with a rust-induced promoter; and

(b) regenerating a plant from the plant cell.

3. The method of claim 1 , wherein the plant is selected from the group consisting of beans, soy, pea, clover, kudzu, lucerne, lentils, lupins, vetches, groundnut, rice, wheat, barley, Arabidopsis , lentil, banana, canola, cotton, potato, corn, sugar cane, alfalfa, and sugar beet.

4. The method of claim 2 , wherein the promoter is a mesophyll-specific promoter having the nucleic acid sequence of SEQ ID NO: 4.

5. A recombinant vector construct comprising in operable linkage:
(a) (i) a nucleic acid comprising SEQ ID NO: 2 or 1; or
(ii) a nucleic acid encoding a CASAR protein having at least 95% identity to SEQ ID NO: 3;

(b) a rust-induced promoter; and
(c) a transcription termination sequence.

6. The vector construct of claim 5 , wherein the promoter is a mesophyll-specific promoter having the nucleic acid sequence of SEQ ID NO: 4.

7. A transgenic soy plant, transgenic soy plant part, or transgenic soy plant cell comprising the recombinant vector construct of claim 5 .

8. A part of the transgenic soy plant of claim 7 , wherein the part comprises the recombinant vector construct.

9. The plant part of claim 8 , wherein the part is a seed comprising the recombinant vector construct.

10. A product derived from the transgenic soy plant of claim 7 , wherein the product comprises the recombinant vector construct.

11. The product of claim 10 , wherein the product is soybean meal or soy oil.

12. A method for the production of a product, said method comprising:
a) growing the transgenic soy plant of claim 7 ; and
b) producing said product from the plant and/or a part thereof.

13. The method of claim 12 , wherein the product is produced from seeds of the plant.

14. The method of claim 12 , wherein the product is soybean meal or soybean oil.

15. A method for breeding a rust resistant plant, said method comprising:
(a) crossing the transgenic soy plant of claim 7 with another soy plant;
(b) obtaining seed from the cross of step (a);
(c) planting said seeds and growing the seeds to plants; and
(d) selecting from the plants produced in step (c) plants expressing the CASAR protein.

16. A method for the production of a transgenic soy plant, transgenic soy plant part, or transgenic soy plant cell having increased resistance against rust, said method comprising:
(a) introducing the recombinant vector construct of claim 5
into a soybean plant, a soybean plant part, or a soybean plant cell; and
(b) regenerating a transgenic plant, transgenic plant part, or transgenic plant cell from the plant, plant part or plant cell.

17. The method of claim 16 , further comprising the step of harvesting the seeds of the transgenic plant and planting the seeds and growing the seeds to plants, wherein the grown plants comprise the recombinant vector construct.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Schultheiss Holger
• Tresch Nadine
• Flachmann Ralf

Classifications

• A23L7/10

  Cereal-derived products · CPC title

• A23L11/07

  Soya beans, e.g. oil-extracted soya bean flakes (A23L11/30 takes precedence) · CPC title

• A23K20/158

  Fatty acids; Fats; Products containing oils or fats · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8282 Primary

  for fungal resistance · CPC title