Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods
US-9499824-B2 · Nov 22, 2016 · US
US9957510B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9957510-B2 |
| Application number | US-201615347936-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 10, 2016 |
| Priority date | Feb 22, 2008 |
| Publication date | May 1, 2018 |
| Grant date | May 1, 2018 |
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Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
Opening claim text (preview).
What is claimed is: 1. An expression vector comprising a nucleic acid encoding a polypeptide having at least 90% sequence identity to SEQ ID NO: 1514 and wherein the polypeptide is a ribose operon repressor. 2. The expression vector of claim 1 , wherein the polypeptide is a central glycolytic genes regulator at a temperature at or above 50 degrees Celsius. 3. The expression vector isolated of claim 1 , wherein the polypeptide has 100% identity to SEQ ID NO: 1514. 4. The expression vector isolated of claim 1 , wherein the nucleic acid has at least 90% identity to SEQ ID NO: 1515. 5. The expression vector isolated of claim 1 , wherein the nucleic acid has 100% identity to SEQ ID NO: 1515. 6. A cell comprising the expression vector of claim 1 . 7. A method of modulating transcription or transcriptional control at temperatures at or above 25 degrees Celsius, the method comprising providing to a transcriptional system the expression vector of claim 1 . 8. The method according to claim 7 , wherein providing to a transcriptional system the expression vector of claim 1 occurs at a temperature at or above 50 degrees Celsius.
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