Method and device for suspending cells

Patent metadata

Field	Value
Publication number	US-9956534-B2
Application number	US-201715676048-A
Country	US
Kind code	B2
Filing date	Aug 14, 2017
Priority date	May 17, 2014
Publication date	May 1, 2018
Grant date	May 1, 2018

Abstract

The invention is related to a device for suspending particles in a fluid, wherein the mixing device includes a first magnet ( 1 ) rotating around a longitudinal axis ( 2 ), a mixing rod ( 4 ) attached to a mount ( 6 ), the mount including a second magnet ( 3 ), wherein the mount ( 6 ) moves in a substantially orthogonal motion to the longitudinal axis of the mixing rod ( 4 ) by the interaction of the rotating first magnet with the second magnet ( 3 ).

First claim

What is claimed is:

1. A mixing device comprising:
a first magnet ( 1 ) rotating around a longitudinal axis ( 2 );
a mixing rod ( 4 ) attached to a mount ( 6 ), the mount including a second magnet ( 3 ), wherein the mount ( 6 ) moves in a substantially orthogonal motion to the longitudinal axis ( 2 ) of the mixing rod ( 4 ) by an interaction of the rotating first magnet with the second magnet ( 3 ) wherein the orthogonal motion is accommodated by a plurality of joints disposed on at least two sides of the mount ( 6 ) and wherein the plurality of joints allows movement of the second magnet ( 3 ) only about a second axis which is orthogonal to the longitudinal axis ( 2 ).

2. The mixing device according to claim 1 , wherein the longitudinal axis ( 2 ) of the first magnet is substantially coaxial with a rotational axis of the second magnet ( 3 ).

3. The mixing device according to claim 1 , characterized in that the mixing rod is a tube.

4. The mixing device according to claim 1 , characterized in that the mixing rod ( 4 ) is coupled to the mount ( 6 ) by a sleeve ( 10 ), wherein the sleeve allows movement of the mixing rod along its longitudinal axis.

5. The mixing device according to claim 1 , characterized in that the mount ( 6 ) is provided with at least one orifice ( 11 , 12 ) which allows introduction of a liquid into a sleeve ( 10 ).

6. The mixing device according to claim 1 , characterized in that the mount ( 6 ) is provided with at least a cleaning pod that removes material from the mixing rod.

7. The mixing device according to claim 1 , characterized in that at least one mixing vessel containing a liquid is provided below the mount ( 6 ), such that the mixing rod is submerged in the liquid so as to stir the liquid by the orthogonal motion.

8. The mixing device according to claim 1 , characterized in that at least one mixing vessel is provided and the mixing rod ( 4 ) is inserted and removed from the mixing vessel by moving the mixing vessel in a direction along the longitudinal axis of the mixing rod ( 4 ).

9. The mixing device according to claim 1 , wherein the mixing rod does not touch walls of a mixing vessel throughout its movement.

10. The mixing device according to claim 7 , wherein the outer diameter of the mixing rod ( 4 ) is between about 0.1 and 0.3 of a diameter of the vessel.

11. The mixing device according to claim 1 , further comprising an additional 1 to 6 mixing rods ( 4 ) and mounts ( 10 ) which are driven in parallel.

12. The mixing device according to claim 1 , wherein the mount ( 6 ) includes a plurality of mixing rods ( 4 ).

13. The mixing device according to claim 1 , further comprising a driver that stops and reverses motion of the mixing rod.

14. The mixing device according to claim 1 , wherein the mixing rod ( 4 ) mixes a biological sample.

Assignees

• Miltenyi Biotec Gmbh
• Miltenyi Biotec Gmbh

Inventors

• Hammerschmidt Dominik
• Ignatius Sven
• Buscher Martin
• Peters Ralf-Peter

Classifications

• B01F2215/0037

  Operations & Transport · mapped topic

• B01F15/00032

  Operations & Transport · mapped topic

• B01F13/0827

  Operations & Transport · mapped topic

• B01F15/0203

  Operations & Transport · mapped topic

• G01N2001/386

  Other diluting or mixing processes · CPC title