Multi-targeting short interfering RNAs

Patent metadata

Field	Value
Publication number	US-9953131-B2
Application number	US-201615273094-A
Country	US
Kind code	B2
Filing date	Sep 22, 2016
Priority date	Jan 29, 2007
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

The present invention relates to novel short interfering RNA (siRNA) molecules that are multi-targeted. More specifically, the present invention relates to siRNA molecules that target two or more sequences. In one embodiment, multi-targeting siRNA molecules are designed to incorporate features of siRNA molecules and features of micro-RNA (miRNA) molecules. In another embodiment, multi-targeting siRNA molecules are designed so that each strand is directed to separate targets.

First claim

What is claimed is:

1. A method for designing a multi-targeting RNA molecule comprising:
(a) selecting one mRNA and one 3′ UTR target sequence;
(b) identifying all 19mer siRNA candidates that have perfect complementarity to the mRNA;
(c) identifying effective cleavage target sites within the mRNA with an siRNA efficacy prediction algorithm; and
(d) ordering the siRNA candidates based on predicted miRNA-like down-regulation;
wherein the multi-targeting RNA molecule targets both the mRNA and the 3′ UTR target sequences.

2. The method of claim 1 , wherein the predicted miRNA-like down-regulation is based on the number of and distance between miRNA-like target sites within the 3′ UTR.

3. The method of claim 1 which further comprises (b′) identifying miRNA-like target sites within the 3′ UTR for each siRNA candidate and removing candidates that have no miRNA-like target sites.

4. The method of claim 2 which further comprises (b′) identifying miRNA-like target sites within the 3′ UTR for each siRNA candidate and removing candidates that have no miRNA-like target sites.

5. The method of claim 1 , wherein the multi-targeting RNA molecule has both siRNA and miRNA functions.

6. The method of claim 1 , wherein the multi-targeting RNA molecule comprises a first strand and a second strand, wherein the first strand targets the mRNA and the second strand targets the 3′ UTR target sequence.

Assignees

• Hope City

Inventors

• Rossi John J
• Snove Ola
• Ehsani Ali
• Saetrom Pal
• Vallazza Britta
• Zhang Jane
• Aagaard Lars

Classifications

• A61P35/04

  specific for metastasis · CPC title

• A61P5/00

  Drugs for disorders of the endocrine system · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61P31/18

  for HIV · CPC title