Dosimeters including lensless imaging systems

Patent metadata

Field	Value
Publication number	US-9952417-B2
Application number	US-201715627866-A
Country	US
Kind code	B2
Filing date	Jun 20, 2017
Priority date	Dec 17, 2013
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

First claim

What is claimed is:

1. A method comprising:
providing a first volume of a sample at a sensor surface of an imaging sensor,
mixing, at the sensor surface of the imaging sensor, at least part of the sample to provide a second volume of the sample at the sensor surface,
capturing an image of the first volume and an image of the second volume by lensless optical microscopy using the imaging sensor, and
based on the images, deriving information related to units present in the first volume and in the second volume.

2. The method of claim 1 in which the information comprises counts of cells of types in blood.

3. The method of claim 2 in which the types of cells include lymphocytes.

4. The method of claim 1 in which the information comprises lymphocyte depletion based on the images.

5. The method of claim 1 , in which the sample comprises a first sample taken at a first time from a subject and the method comprises:
repeating the providing, mixing, capturing, and deriving activities for a second sample taken at a second, different time from the subject, and
estimating lymphocyte depletion.

6. The method of claim 1 in which the sample contains fiduciary beads.

7. The method of claim 2 in which cells of different types are differentiated based on one or more of color, size of cell, nuclear shape, and nuclear size.

8. The method of claim 2 in which the counts are corrected for a volume of the imaged sample.

9. The method of claim 2 in which the sample contains diluted blood from the subject, and the counts are corrected for dilution of the blood.

10. The method of claim 1 in which the sample contains one or more of anticoagulant, diluent, stain, antibody, erythrocyte lysing solution, and other reagents.

11. The method of claim 2 in which the counts are based on detection of one or more surface antigens.

12. The method of claim 1 in which the images are captured at a resolution of 1 mega pixels or higher.

13. The method of claim 1 in which the images contain information about units distributed in no more than a monolayer in the sample.

14. The method of claim 1 in which the mixing comprises remixing.

15. The method of claim 1 in which the mixing comprises resampling.

16. The method of claim 1 in which the sample comprises a bodily fluid of a subject.

17. The method of claim 16 in which the sample comprises blood of a subject.

18. The method of claim 1 in which the information comprises statistical information.

19. The method of claim 1 in which the first volume and the second volume contain units that are captured in the images and at least some of the units in the first volume are different from some of the units in the second volume.

20. The method of claim 1 in which the mixing comprises moving a mixing element relative to the sensor surface.

21. The method of claim 20 in which moving of the mixing element comprises moving the mixing element toward or away from the sensor surface or both.

22. The method of claim 20 in which the moving of the mixing element comprises moving the mixing element toward and away from the sensor surface more than one time.

23. The method of claim 22 in which the moving of the mixing element is done rapidly.

24. The method of claim 20 in which the mixing element comprises a mixing surface.

25. The method of claim 24 in which the mixing surface is part of a chamber top.

26. The method of claim 20 in which the mixing element is in contact with the part of the sample.

27. The method of claim 1 in which the information related to the units comprises information related to at least one type of the units in the sample.

28. The method of claim 1 in which the information related to the units comprises a radiation dose absorbed by a subject from whom the sample was obtained.

29. The method of claim 1 in which the units comprise blood cells.

Assignees

• Alentic Microscience Inc

Inventors

• Fine Alan Marc

Classifications

• G02B21/361

  Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups) · CPC title

• G03H2001/005

  in microscopy, e.g. digital holographic microscope [DHM] · CPC title

• G02B21/365

  Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title

• G01B9/02041

  characterised by particular imaging or detection techniques · CPC title

• G01N15/1429

  Signal processing · CPC title