Method for detecting albumin based on colorimetric assay and system thereof

Patent metadata

Field	Value
Publication number	US-9952196-B2
Application number	US-201514985986-A
Country	US
Kind code	B2
Filing date	Dec 31, 2015
Priority date	Nov 23, 2015
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

A method for detecting albumin based on a colorimetric assay and a system thereof are disclosed. Gold nanoparticles are added into the sample preparing device having a sample without spectroscopic tags, wherein the sample without spectroscopic tags is formed as the alkaline solution to avoid the interference substances adhering on the gold nanoparticles. The gold nanoparticles are concentrated by using the microfluidic concentrator with the circular ion exchange membrane by applying an external electric field across two electrodes. The image of the concentrated gold nanoparticles is captured by the image capturing device for measuring the saturation intensities of the image, wherein there is a relation between the saturation intensities and the concentration of the albumin in the sample without spectroscopic tags. The concentration of the albumin of the sample without spectroscopic tags is obtained by the relation and the measured saturation intensities.

First claim

What is claimed is:

1. A method for detecting albumin based on a colorimetric assay, comprising:
adding gold nanoparticles into a sample preparing device having a sample without spectroscopic tags, preparing the sample without spectroscopic tags to form an alkaline solution to avoid interference substances adhering on the gold nanoparticles;
concentrating the gold nanoparticles by using a microfluidic concentrator with a circular ion exchange membrane and by applying an external electric field across two electrodes;
capturing an image of the concentrated gold nanoparticles by an image capturing device for measuring saturation intensities of the image, wherein there is a relation between the saturation intensities and a concentration of the albumin in the sample without spectroscopic tags; and
obtaining the concentration of the albumin of the sample without spectroscopic tags by using the relation and the measured saturation intensities.

2. The method for detecting albumin based on the colorimetric assay of claim 1 , wherein the saturation intensities comprise a saturation intensity difference among three primary colors of the image.

3. The method for detecting albumin based on the colorimetric assay of claim 2 , wherein the relation denotes a linear relation between the saturation intensity difference and a logarithm of the concentration of the albumin.

4. The method for detecting albumin based on the colorimetric assay of claim 1 , wherein a pH value of the alkaline solution is substantially 10.

5. The method for detecting albumin based on the colorimetric assay of claim 1 , wherein the image capturing device is a charge coupled device or a complementary metal oxide semiconductor image sensor built in a mobile device.

6. A system of detecting albumin based on a colorimetric assay, comprising:
a sample preparing device having a sample without spectroscopic tags, gold nanoparticles added into the sample preparing device, wherein the sample without spectroscopic tags are prepared to form an alkaline solution to avoid interference substances adhering on the gold nanoparticles when the gold nanoparticles are added into the sample preparing device having the sample without spectroscopic tags;
a microfluidic concentrator concentrating the gold nanoparticles by using a circular ion exchange membrane and by applying an external electric field across two electrodes;
an image capturing device capturing an image of the concentrated gold nanoparticles for measuring saturation intensities of the image, wherein there is a relation between the saturation intensities and a concentration of the albumin in the sample without spectroscopic tags; and
a processing device, the image capturing device transmitting the saturation intensities to the processing device, and the processing device obtaining the concentration of the albumin of the sample without spectroscopic tags by using the relation and the measured saturation intensities.

7. The system of detecting albumin based on the colorimetric assay of claim 6 , further comprising a storage storing the relation.

8. The system of detecting albumin based on the colorimetric assay of claim 6 , wherein the saturation intensities comprise a saturation intensity difference among three primary colors of the image, and the relation denotes a linear relation between the saturation intensity difference and a logarithm of the concentration of the albumin.

9. The system of detecting albumin based on the colorimetric assay of claim 6 , wherein a pH value of the alkaline solution is substantially 10.

10. The system of detecting albumin based on the colorimetric assay of claim 6 , wherein the image capturing device is a charge coupled device or a complementary metal oxide semiconductor image sensor built in a mobile device.

Assignees

• Univ Nat Chung Cheng

Inventors

• Wang Shau-Chun
• Lai Teh-Sheng
• Chang Ting-Chou

Classifications

• G01N2001/4011

  being a ion-exchange membrane · CPC title

• G01N33/6827

  Total protein determination, e.g. albumin in urine · CPC title

• G01N1/4005

  by transferring a selected component through a membrane · CPC title

• B01L3/50

  Containers for the purpose of retaining a material to be analysed, e.g. test tubes (devices for taking samples of blood A61B5/15) · CPC title

• G01N21/554

  detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance · CPC title