Methods, systems, and computer readable media for determining physical properties of a specimen in a portable point of care diagnostic device

Patent metadata

Field	Value
Publication number	US-9952149-B2
Application number	US-201314648845-A
Country	US
Kind code	B2
Filing date	Dec 2, 2013
Priority date	Nov 30, 2012
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

Methods, systems, and computer readable media for determining physical properties of a specimen in a portable point of care device are disclosed. According to one aspect, a method includes placing a specimen onto an active surface that includes a plurality of microposts extending outwards from a substrate, wherein each micropost includes a proximal end attached to the substrate and a distal end opposite the proximal end and generating an actuation force in proximity to the micropost array that compels at least some of the microposts to exhibit motion. The method further includes detecting light that is emitted by an illumination source and interacts with the active surface while the at least some microposts exhibit motion in response to the actuation force, measuring data that represents the detected light interacting with the active surface, and determining at least one physical property of the specimen based on the measured data.

First claim

What is claimed is:

1. A method comprising:
receiving, by a portable point of care device, a specimen applied to an active surface that includes a micropost array comprising a plurality of microposts extending outwards from a substrate, wherein each micropost includes a proximal end attached to the substrate and a distal end opposite the proximal end, and a metallic, ferromagnetic or ferroelectric material on at least a portion of some of the microposts;
generating, by the portable point of care device, an actuation force in proximity to the micropost array that compels at least some of the microposts to exhibit motion, wherein the actuation force is applied in accordance with a varying actuation force amplitude or a varying actuation force frequency;
detecting light that is emitted by an illumination source within the portable point of care device and that interacts with the active surface while the at least some microposts exhibit motion in response to the actuation force;
measuring data that represents the detected light interacting with the active surface; and
determining at least one physical property of the specimen based on the measured data and feedback data pertaining to either the varying actuation force amplitude or the varying actuation force frequency, wherein the feedback data is used to adjust the amplitude or frequency of the actuation force.

2. The method of claim 1 wherein a reagent is added to the specimen prior to applying the specimen to the active surface or the reagent is pre-applied to the active surface as a liquid or solid.

3. The method of claim 1 wherein the specimen comprises blood and the physical property comprises a clotting property, a lysis property, a platelet function property, or a clot stiffness property of the blood.

4. The method of claim 1 wherein the actuation force comprises an electric or magnetic force.

5. The method of claim 1 wherein the actuation force is applied to the at least some of the microposts to manipulate, mix, or alter the specimen.

6. The method of claim 1 wherein the generated actuation force is able to be switched from among a plurality of different actuation force modes, including one or more of a manipulation mode, a measurement mode, a mixing mode, and a detection method mode.

7. A system comprising:
an active surface configured to receive a specimen and that includes a micropost array comprising a plurality of microposts extending outwards from a substrate, wherein each micropost includes a proximal end attached to the substrate, a distal end opposite the proximal end, and a metallic, ferromagnetic or ferroelectric material on at least a portion of some of the microposts;
an actuation unit in a portable point of care device configured to generate an actuation force in proximity to the micropost array that compels at least some of the microposts to exhibit motion when the active surface is received by the portable point of care device, wherein the actuation force is applied in accordance with a varying actuation force amplitude or a varying actuation force frequency;
an illumination source in the portable point of care device configured to emit light;
a detection unit in the portable point of care device configured to detect the light that is emitted by the illumination source and interacts with the active surface while the at least some microposts exhibit motion in response to the actuation force; and
a processing unit in the portable point of care device configured to measure data that represents the detected light interacting with the active surface and to determine at least one physical property of the specimen based on the measured data and feedback data pertaining to either the varying actuation force amplitude or the varying actuation force frequency, wherein the feedback data is used to adjust the amplitude or frequency of the actuation force.

8. The system of claim 7 wherein a reagent is added to the specimen prior to the active surface receiving the specimen or the reagent is pre-applied to the active surface as a liquid or solid.

9. The system of claim 7 wherein the specimen comprises blood and the physical property comprises a clotting property, a lysis property, a platelet function property, or a clot stiffness property of the blood.

10. The system of claim 7 wherein the actuation force comprises an electric or magnetic force.

11. The system of claim 7 wherein the actuation force is applied to the at least some of the microposts to manipulate, mix, or alter the specimen.

12. The system of claim 7 wherein the generated actuation force is able to be switched from among a plurality of different actuation force modes, including at least one of a manipulation mode, a measurement mode, a mixing mode, and a detection method mode.

Assignees

• Univ North Carolina Chapel Hill

Inventors

• Superfine Richard
• Oberhardt Bruce J
• Spero Richard Chasen
• Falvo Michael Richard
• Fiser Briana Lee
• Taylor Ii Russell Morton
• Judith Robert Michael

Classifications

• G01N33/4905

  Determining clotting time of blood (by chemical methods G01N33/86, C12Q1/56) · CPC title

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N2021/825

  Agglutination · CPC title

• G01N2201/02

  Mechanical · CPC title

• G01N2201/062

  LED's · CPC title