Animal feed enzyme extraction
US-2015361478-A1 · Dec 17, 2015 · US
Probes and assays for measuring E3 ligase activity
US9951371B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9951371-B2 |
| Application number | US-201514856251-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 16, 2015 |
| Priority date | Sep 17, 2014 |
| Publication date | Apr 24, 2018 |
| Grant date | Apr 24, 2018 |
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- Title
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Abstract
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Provided herein is technology relating to the biological process of protein ubiquitination and particularly, but not exclusively, to compositions and methods for studying protein ubiquitination and developing therapeutics to modulate protein ubiquitination.
First claim
Opening claim text (preview).
We claim: 1. A composition comprising a ubiquitin C-terminal thioester fluorophore. comprises a ubiquitin covalently attached to a fluorophore by a thioester. 2. The composition of claim 1 wherein the ubiquitin C-terminal thioester fluorophore comprises a ubiquitin covalently attached to a linker and a linker covalently attached to the fluorophore. 3. The composition of claim 1 wherein the fluorophore is Fluorescein, Rhodamine, BODIPY, Alexa Fluor 488, Oregon Green 488, or Alexa Fluor 594. 4. The composition of claim 2 wherein the linker is an alkyl, cycloalkyl, aryl, heteroaryl, heteroalkyl polymer, carbon nanotube, quantum dot, or nanoparticle. 5. The composition of claim 1 wherein the ubiquitin comprises amino acids 1-76 of the ubiquitin polypeptide (SEQ ID NO: 1). 6. The composition of claim 1 further comprising an E3 ligase. 7. The composition of claim 6 wherein the E3 ligase is NEDD4, NEDD4L, ITCH, WWP1, WWP2, SMURF1, SMURF2, NEDL1, NEDL2, E6AP, HECTD2, KIAA0614, TRIP12, G2E3, EDD, HACE1, HECTD1, UBE3B, UBE3C, KIAA0317, HUWE1, HECTD3, HERC1, HERC2, HERC3, HERC4, HERC5, HERC6, SopA, NleL, ARIH1, ARIH2, CUL9, ANKIB1, PARK2, RNF144A, RNF144B, RBCK1, RNF19A, RNF19B, RNF31, RNF216, RNF14, RNF217, or a NEL E3 ligase. 8. The composition of claim 6 further comprising free fluorophore. 9. The composition of claim 6 further comprising activated E3˜Ub thioester.
Assignees
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Classifications
with steric inhibition or signal modification, e.g. fluorescent quenching · CPC title
- C12Q1/25Primary
involving enzymes not classifiable in groups C12Q1/26 {- C12Q1/66} · CPC title
Ligases (6) · CPC title
Ubiquitin-protein ligase (6.3.2.19), i.e. ubiquitin-conjugating enzyme · CPC title
from mammals · CPC title
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Frequently asked questions
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- What does patent US9951371B2 cover?
- Provided herein is technology relating to the biological process of protein ubiquitination and particularly, but not exclusively, to compositions and methods for studying protein ubiquitination and developing therapeutics to modulate protein ubiquitination.
- Who is the assignee on this patent?
- Univ Northwestern
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/25. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 24 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).