Fiber-reinforced hydrogel composites and methods of forming fiber-reinforced hydrogel composites

Patent metadata

Field	Value
Publication number	US-9950093-B2
Application number	US-201314401014-A
Country	US
Kind code	B2
Filing date	May 15, 2013
Priority date	May 15, 2012
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

A fiber-reinforced hydrogel composite is provided. The composite includes a hydrogel and a fibrous component containing a plurality of fibers. Length of each of the plurality of fibers is less than about 1,000 μm. A method of preparing a fiber-reinforced hydrogel composite is also provided. The method includes coating a hydrogel precursor solution on a substrate to form a hydrogel precursor film, depositing the plurality of fibers onto the hydrogel precursor film, and allowing the hydrogel precursor film to form a hydrogel film, thereby forming the fiber-reinforced hydrogel composite. A scaffold containing the fiber-reinforced composite, and a tissue repair method using the fiber-reinforced composite are also provided.

First claim

The invention claimed is:

1. A fiber-reinforced hydrogel composite comprising:
a hydrogel; and
a fibrous component comprising a plurality of fibers, wherein the fibers comprise albumin, collagen, elastin, alginate, polylactide, polyamide, poly(siloxane), poly(silicone), poly(ethylene), poly(vinyl pyrrolidone), poly(2-hydroxy ethyl methacrylate), poly(N-vinyl pyrrolidone), poly(methyl methacrylate), poly(vinyl alcohol), poly(acrylic acid), poly(vinyl acetate), polyacrylamide, poly(ethylene-co-vinyl acetate), poly(ethylene glycol), poly(methacrylic acid), polyglycolide, poly(lactide-co-glycolide), polyanhydride, polyorthoester, poly(carbonate), poly(ethylene oxide), polyaniline, poly(vinyl phenol), polyhydroxyacid, poly(caprolactone), polyhydroxyalkanoate, polyurethane, chitosan, starch, hyaluronic acid, blends thereof, or copolymers thereof, and wherein the length of each of the plurality of fibers is less than about 1,000 um; wherein the fiber-reinforced hydrogel is a molded fiber-reinforced hydrogel.

2. The composite of claim 1 , wherein the length of each of the plurality of fibers is about 100 μm to about 1,000 μm.

3. The composite of claim 1 , wherein the plurality of fibers consists of electrospun fibers.

4. The composite of claim 1 , wherein the composite consists of less than about 10 wt % of the fibrous component based on the total weight of the composite.

5. The composite of claim 4 , wherein the composite consists of about 1 wt % to about 6 wt % of the fibrous component based on the total weight of the composite.

6. The composite of claim 1 , comprising a plurality of layers of the hydrogel and/or a plurality of layers of the fibrous component.

7. The composite of claim 6 , wherein the composite consists of alternating layers of the hydrogel and the fibrous component.

8. The composite of claim 1 , wherein the fibers consist of serum albumin.

9. The composite of claim 8 , wherein the fibers consist of bovine serum albumin.

10. The composite of claim 8 , wherein the hydrogel comprises dextran dialdehyde-crosslinked gelatin.

11. The composite of claim 1 , wherein the hydrogel comprises one or more synthetic or natural hydrophilic polymers.

12. The composite of claim 11 , wherein the hydrogel comprises one or more materials selected from the group consisting of polysaccharides, proteins, polyethylene glycol, polylactic acid, polycaprolactone, polyglycolide, and combinations thereof.

13. The composite of claim 12 , wherein the hydrogel comprises one or more compounds selected from the group consisting of dextran, chitosan, hyaluronic acid, gelatin, dextran dialdehyde-crosslinked gelatin, collagen, aminated hyaluronic acid, hyaluronic acid-g-poly(N-isopropylacrylamide), chitosan-hyaluronic acid, laminin, elastin, alginate, fibronectin, polyethylene glycolfibrinogen and derivatives thereof.

14. The composite of claim 13 , wherein the hydrogel comprises dextran dialdehyde-crosslinked gelatin.

15. The composite of claim 1 , wherein the material of the hydrogel and the material of the fibers are different.

16. A scaffold having a three-dimensional shape, comprising a fiber-reinforced hydrogel composite including: a hydrogel; and a fibrous component comprising a plurality of fibers, wherein the fibers comprise albumin, collagen, elastin, alginate, polylactide, polyamide, poly(siloxane), poly(silicone), poly(ethylene), poly(vinyl pyrrolidone), poly(2-hydroxy ethyl methacrylate), poly(N-vinyl pyrrolidone), poly(methyl methacrylate), poly(vinyl alcohol), poly(acrylic acid), poly(vinyl acetate), polyacrylamide, poly(ethylene-co-vinyl acetate), poly(ethylene glycol), poly(methacrylic acid), polyglycolide, poly(lactide-co-glycolide), polyanhydride, polyorthoester, poly(carbonate), poly(ethylene oxide), polyaniline, poly(vinyl phenol), polyhydroxyacid, poly(caprolactone), polyhydroxyalkanoate, polyurethane, chitosan, starch, hyaluronic acid, blends thereof, or copolymers thereof, and wherein the length of each of the plurality of fibers is less than about 1,000 um; wherein the fiber-reinforced hydrogel is a molded fiber-reinforced hydrogel.

Assignees

• Technion Res & Development Found Ltd
• Nat Univ Singapore

Inventors

• Zussman Eyal
• Chaganti Srinivasa Reddy
• Venugopal Jayarama Reddy
• Ramakrishna Seeram
• Regev Omri

Classifications

• A61L2400/06

  Flowable or injectable implant compositions · CPC title

• A61L27/48

  with macromolecular fillers · CPC title

• Y10T428/31978

  Cellulosic next to another cellulosic · CPC title

• A61F2/02

  Prostheses implantable into the body · CPC title

• A61L27/26 Primary

  Mixtures of macromolecular compounds · CPC title