Methods for rejuvenating red blood cells

Patent metadata

Field	Value
Publication number	US-9950012-B2
Application number	US-201514748565-A
Country	US
Kind code	B2
Filing date	Jun 24, 2015
Priority date	Jan 31, 2013
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

Methods and a system for transfusing blood to a mammalian subject. The method includes obtaining a volume of donated red blood cells (RBCs), adding an enhancement composition to the RBCs to form a treated blood composition, incubating the treated blood composition to form an incubated blood composition, and administering the incubated blood composition to a patient. The system includes a functionally-closed, sterile Y-type tube set.

First claim

What is claimed is:

1. A method for transfusing blood to a mammalian subject, the method comprising, the steps of:
fluidly connecting a container of red blood cells to an output of a Y-type connector of a sterile, closed processing system;
fluidly connecting a container comprising an enhancement composition to a first input of the Y-type connector of the closed processing system, the enhancement composition comprising inosine, adenine, pyruvate, sodium phosphate, or a combination thereof;
flowing the enhancement composition through a filter of the closed processing system and into the container of red blood cells to form a treated red blood cell composition; and
incubating the treated red blood cell composition, forming an incubated red blood cell composition comprising treated red blood cells;
fluidly connecting a transfer bag to a second input of the Y-type connector, the transfer bag comprising a wash solution;
washing the treated red blood cells, including flowing the wash solution through the Y-type connector and into the treated red blood cells; and
administering the treated red blood cells to the subject after the washing step.

2. The method of claim 1 , wherein the enhancement composition comprises:
from about 25 g/L to about 30 g/L of inosine;
from about 0.2 g/L to about 2 g/L of adenine;
from about 5 g/L to about 15 g/L of pyruvate;
from about 17 g/L to about 23 g/L of sodium phosphate;

or a combination thereof.

3. The method of claim 1 , wherein the enhancement composition comprises about 27 g/L inosine, about 11 g/L pyruvate, about 0.7 g/L adenine, and about 21 g/L phosphate mixture, and has a pH from about 6.5 to about 7.5.

4. The method of claim 1 , wherein the container of red blood cells was stored at a preservation temperature prior to the fluidly connecting step.

5. The method of claim 1 , further comprising separating the treated red blood cells from the enhancement composition before the administering step.

6. The method of claim 5 , wherein separating the treated red blood cells from the enhancement composition includes flowing the enhancement composition through the Y-type connector and into the transfer bag.

7. The method of claim 1 , further comprising separating the wash solution from the treated red blood cells prior to the administering step.

8. The method of claim 1 , wherein the incubating is from about 30 minutes to about 90 minutes at a temperature of from about 35° C. to about 40° C.

9. The method of claim 1 , further comprising processing the incubated red blood cell composition to form a pellet of treated red blood cells.

10. The method of claim 9 , wherein the transfer bag further includes an additive solution, separated from the wash solution, the method further comprising flowing the additive solution through the Y-type connector and into the container to suspend the pellet of treated red blood cells in the additive solution.

11. A method for improving the metabolic function of blood, comprising:
fluidly connecting a container comprising a volume of red blood cells to a sterile, closed processing system comprising a first tube configured for fluid communication with the container, a first transfer bag and a second transfer bag contemporaneously coupled to a second tube of the closed processing system;
fluidly connecting an enhancement composition to the closed processing system, including adding the enhancement composition to the volume of red blood cells to form a treated blood composition;
incubating the treated blood composition to form an incubated red blood cell composition; and
processing the incubated red blood cell composition to separate the red blood cells and a fluid comprising the enhancement composition, the red blood cells having an enhanced metabolic function.

12. The method of claim 11 , wherein the first transfer bag comprises a wash solution, the method further comprising diluting the incubated red blood cell composition with the wash solution prior to the processing step.

13. The method of claim 11 , wherein processing includes collecting the fluid in the first transfer bag or the second transfer bag.

14. The method of claim 11 , wherein the second transfer bag has an additive solution, and wherein processing includes collecting the fluid in the first transfer bag and flowing the additive solution into the separated red blood cells after collecting the fluid in the first transfer bag.

15. The method of claim 11 , wherein the enhancement composition comprises:
from about 25 g/L to about 30 g/L of inosine;
from about 0.2 g/L to about 2 g/L of adenine;
from about 5 g/L to about 15 g/L of pyruvate;
from about 17 g/L to about 23 g/L of sodium phosphate; or
a combination thereof.

16. The method of claim 11 , wherein the enhancement composition comprises:
from about 25 g/L to about 30 g/L of inosine;
from about 0.2 g/L to about 2 g/L of adenine;
from about 5 g/L to about 15 g/L of pyruvate; and
from about 17 g/L to about 23 g/L of sodium phosphate.

17. The method of claim 11 , wherein the enhanced metabolic function increases the concentration of 2,3-diphosphoglycerate, adenosine triphosphate, or both, in the red blood cells.

18. The method of claim 11 , further comprising administering to a subject the red blood cells having an enhanced metabolic function.

19. The method of claim 18 , wherein the subject is experiencing sepsis, septic shock, Upper Gastrointestinal Bleeding (UGIB), anemia, severe trauma, heart attack or stroke.

Assignees

• Biomet Biologics Llc

Inventors

• Gray Alan

Classifications

• A61M1/0218

  with filters · CPC title

• A61M1/0272

  Apparatus for treatment of blood or blood constituents prior to or for conservation, e.g. freezing, drying or centrifuging · CPC title

• A61M1/3693

  using separation based on different densities of components, e.g. centrifuging · CPC title

• A61M1/0281

  Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing · CPC title

• A61K35/18 Primary

  Erythrocytes (haemoglobin A61K38/42) · CPC title