AAD-12 event 416, related transgenic soybean lines, and event-specific identification thereof

Patent metadata

Field	Value
Publication number	US-9944944-B2
Application number	US-201013511990-A
Country	US
Kind code	B2
Filing date	Nov 24, 2010
Priority date	Nov 24, 2009
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

This invention includes a novel aad-12 transformation event for herbicide tolerance in soybean plants—referred to herein as pDAB4468-0416. This invention includes a heterologous polynucleotide inserted into a specific site within the genome of a soybean cell. In some embodiments, said event/polynucleotide can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (a soybean, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

First claim

The invention claimed is:

1. A transgenic soybean plant comprising a single copy of residues 2731 to 9121 of SEQ ID NO:1, inserted into the genome of said soybean plant between a host sequence of residues 1 to 2730 of SEQ ID NO:1 and a host sequence of residues 9122 to 10,212 of SEQ ID NO:1.

2. A soybean seed comprising a genome comprising AAD-12 event pDAB4468-0416 as present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-10442.

3. A soybean seed from the plant of claim 1 , said seed comprising SEQ ID NO:1.

4. A soybean plant produced by growing the seed of claim 2 , said plant comprising SEQ ID NO:1.

5. A progeny plant of the soybean plant of claim 4 , said progeny plant comprising SEQ ID NO:1.

6. A herbicide-tolerant progeny plant of the soybean plant of claim 1 , said progeny plant comprising SEQ ID NO:1.

7. A part of the plant of claim 4 wherein said part is selected from the group consisting of pollen, ovule, flowers, shoots, roots, and leaves, said part comprising SEQ ID NO:1.

8. An isolated polynucleotide molecule wherein said molecule comprises at least 15 nucleotides and hybridizes under stringent wash conditions with a nucleotide sequence selected from the group consisting of residues 2720 to 2740 of SEQ ID NO:1, residues 9112 to 9132 of SEQ ID NO:1, and complements thereof.

9. A method of breeding a soybean plant, said method comprising crossing the soybean plant of claim 1 with a second soybean plant to produce a third soybean plant comprising a genome, and assaying said third soybean plant for presence of SEQ ID NO:1 in said genome.

10. A method of introgressing a herbicide tolerance trait into a soybean plant, said method comprising crossing the soybean plant of claim 1 with a second soybean plant to produce a third soybean plant comprising a genome, and assaying said third soybean plant for presence of SEQ ID NO:1 in said genome.

11. A method of controlling weeds, said method comprising applying an aryloxy alkanoate herbicide to a field, said field comprising a plant of claim 1 .

12. The method of claim 11 , wherein said herbicide is selected from the group consisting of 2,4-D; 2,4-DB; MCPA; and MCPB.

13. The method of claim 11 , wherein said method comprises applying a second herbicide to said field.

14. The method of claim 13 , wherein said second herbicide is selected from the group consisting of glyphosate and dicamba.

15. A method of controlling weeds, said method comprising applying an aryloxy alkanoate herbicide to a field, and planting a seed of claim 3 in said field within 14days of applying the herbicide.

16. A method of detecting a soybean event in a sample comprising soybean DNA wherein said method comprises contacting said sample with at least one polynucleotide that is diagnostic for the AAD-12 event as present in the seed of claim 2 .

17. The method of claim 16 wherein said method comprises contacting said sample with
a. a first primer that binds to a flanking sequence selected from the group consisting of residues 1-2730 of SEQ ID NO:1, residues 9122-10,212 of SEQ ID NO:1, and complements thereof; and
b. a second primer that binds to an insert sequence comprising residues 2731-9121 of SEQ ID NO:1 or the complement thereof;
subjecting said sample to polymerase chain reaction; and
assaying for an amplicon generated between said primers.

18. The method of claim 16 wherein said at least one polynucleotide comprises at least 30 nucleotides and hybridizes under stringent conditions with a sequence selected from the group consisting of residues 2720 to 2740 of SEQ ID NO:1, residues 9112 to 9132 of SEQ ID NO:1, and complements thereof; wherein said method further comprises subjecting said sample and said polynucleotide to stringent hybridization conditions; and assaying said sample for hybridization of said polynucleotide to said DNA.

19. The method of claim 11 wherein said method is used to treat or prevent herbicide-resistant weeds.

20. The method of claim 11 wherein said herbicide is applied prior to planting said plant.

21. A method of controlling glyphosate-resistant weeds in an area comprising at least one plant of claim 1 , wherein said plant further comprises a glyphosate tolerance trait, and said method comprises applying an aryloxyalkanoate herbicide to at least a portion of said area.

22. The method of claim 21 wherein said herbicide is a phenoxy auxin.

23. The method of claim 21 wherein said herbicide is applied from a tank mix with glyphosate.

24. The method of claim 11 wherein at least one of said weeds is a glyphosate-resistant volunteer of a different species than said plant.

Assignees

• Cui Yunxing C
• Hoffman Thomas
• Zhou Ning
• Gilles Gregory J
• Wright Terry R
• Colon Julissa
• Barnes Ricardo A
• Vanopdorp Nathan J
• Bai Yonghe
• Dow Agrosciences Llc

Inventors

• Cui Yunxing C
• Hoffman Thomas
• Zhou Ning
• Gilles Gregory J
• Wright Terry R
• Colon Julissa
• Barnes Ricardo A
• Vanopdorp Nathan J
• Bai Yonghe

Classifications

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• A01H5/00 Primary

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title