Methods and compositions for enhanced resistance to abiotic stress in plants

Patent metadata

Field	Value
Publication number	US-9944942-B2
Application number	US-201213672320-A
Country	US
Kind code	B2
Filing date	Nov 8, 2012
Priority date	Nov 8, 2011
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

The present invention provides transgenic plants having increased tolerance to abiotic stress comprising a recombinant nucleic acid molecule, said recombinant nucleic acid molecule comprising a nucleotide sequence encoding miR319 operatively associated with a promoter, a nucleotide sequence that is antisense to a portion of consecutive nucleotides of a nucleotide sequence encoding PCF5, and/or a nucleotide sequence that encodes a portion of consecutive nucleotides of a nucleotide sequence encoding PCF5, which when expressed produces an antisense nucleotide sequence, wherein expression of the nucleotide sequence confers increased tolerance to abiotic stress. Also provided are methods and compositions for making said transgenic plants.

First claim

What is claimed is:

1. A transgenic plant having increased tolerance to drought, the plant comprising a recombinant nucleic acid molecule, said recombinant nucleic acid molecule comprising a nucleotide sequence encoding miR319 operatively associated with a promoter, wherein overexpression of the nucleotide sequence encoding miR319 confers increased tolerance to drought, and wherein the nucleotide sequence encoding miR319 is the nucleotide sequence of SEQ ID NO: 1, the nucleotide sequence of SEQ ID NO: 7, and/or the nucleotide sequence of SEQ ID NO: 8.

2. A method of producing a transgenic plant having increased tolerance to drought, the method comprising:
a) transforming a plant cell with a recombinant nucleic acid molecule, wherein said recombinant nucleic acid molecule comprises
a nucleotide sequence encoding miR319 operatively associated with a promoter, wherein the nucleotide sequence encoding miR319 is the nucleotide sequence of SEQ ID NO: 1, the nucleotide sequence of SEQ ID NO: 7, and/or the nucleotide sequence of SEQ ID NO: 8; and

(b) regenerating a transgenic plant from the transformed plant cell, thereby producing a transgenic plant having increased tolerance to drought.

3. A transgenic plant having increased tolerance to drought produced by the method of claim 2 .

4. A seed of the transgenic plant of claim 1 , wherein the seed comprises said recombinant nucleic acid molecule in its genome.

5. A seed of the transgenic plant of claim 3 , wherein the seed comprises said recombinant nucleic acid molecule in its genome.

6. A crop comprising a plurality of transgenic plants of claim 1 , planted together in an agricultural field, a golf course, a residential lawn, a road side, an athletic field, and/o a recreational field.

7. A crop comprising a plurality of transgenic plants of claim 3 , planted together in an agricultural field, a golf course, a residential lawn, a road side, an athletic field, and/or a recreational field.

Assignees

• Univ Clemson

Inventors

• Luo Hong
• Li Dayong
• Zhou Man
• Hu Qian

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title