Chelator and use thereof

Patent metadata

Field	Value
Publication number	US-9944722-B2
Application number	US-201113701074-A
Country	US
Kind code	B2
Filing date	May 30, 2011
Priority date	Jun 1, 2010
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.
The dimeric pentadentate chelator according to the invention has the following general formula:

wherein
Sc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), and
PD is a pentadentate chelator having the formula:

First claim

The invention claimed is:

1. A dimeric pentadentate chelator having the following general formula:

wherein PD is a pentadentate chelator having the formula:

wherein S p1 is 6-amino hexanoic acid;
wherein Sc is diethylenetriamine;
wherein an amine of S p1 is coupled to PD to form an amide bond; and
wherein a carboxyl of S p1 is coupled to a primary amine of Sc to form an amide bond.

2. The dimeric pentadentate chelator of claim 1 , further comprising a group Q and having the following general formula:

wherein Q is selected from a solid phase, a detection label, and a biomarker.

3. The dimeric pentadentate chelator of claim 2 , wherein Q is a solid phase comprising a natural or synthetic polymer.

4. The dimeric pentadentate chelator of claim 2 , wherein the solid phase is a porous chromatographic support.

5. The dimeric pentadentate chelator of claim 3 , wherein Q is made of a cross-linked carbohydrate material selected from the group consisting of agarose, agar, cellulose, dextran, chitosan, konjac, carrageenan, gellan and alginate.

6. The dimeric pentadentate chelator of claim 3 , wherein Q is made of synthetic polymers selected from the group consisting of styrene derivatives, divinylbenzene, acryl amides, acrylate esters, methacrylate esters, vinyl esters and vinyl amides.

7. The dimeric pentadentate chelator of claim 2 , wherein Q comprises magnetic particles.

8. The dimeric pentadentate chelator of claim 2 , wherein Q is a solid phase comprising a sensor surface.

9. The dimeric pentadentate chelator of claim 5 , wherein Q is made of agarose.

Assignees

• Algotsson Mattias
• Andersson Lars C
• Brekkan Eggert
• Hedemyr Lee
• Maloisel Jean Luc
• Palmgren Ronnie
• Ge Healthcare Bioprocess R&D Ab

Inventors

• Algotsson Mattias
• Andersson Lars C
• Brekkan Eggert
• Hedemyr Lee
• Maloisel Jean-Luc
• Palmgren Ronnie

Classifications

• C08B37/0039 Primary

  Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose · CPC title

• B01J20/3265

  with an organic functional group containing a metal, e.g. a metal affinity ligand · CPC title

• B01J20/3251

  comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur · CPC title

• B01J20/281

  Sorbents specially adapted for preparative, analytical or investigative chromatography · CPC title

• C07C237/10

  having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by nitrogen atoms not being part of nitro or nitroso groups · CPC title