Detector and method for detecting an agent in an aerosol

Patent metadata

Field	Value
Publication number	US-9943845-B2
Application number	US-201615338024-A
Country	US
Kind code	B2
Filing date	Oct 28, 2016
Priority date	Oct 28, 2015
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

A detector for detecting an agent within an aerosol is provided. The detector may include a liquid feeder configured to generate a droplet comprised of an assay reagent. The detector may further include an aerosol focuser configured to capture and focus the aerosol such that the aerosol is configured to be encapsulated within the droplet in order to cause the assay reagent to react in the droplet. The detector may even further include an interrogator configured to interrogate the droplet in order to detect the agent within the aerosol.

First claim

What is claimed is:

1. A detector for detecting an agent within an aerosol, the detector comprising:
a liquid feeder configured to generate a droplet comprised of an assay reagent;
an aerosol focuser configured to capture and focus the aerosol such that the aerosol is configured to be encapsulated within the droplet in order to cause the assay reagent to react in the droplet; and
an interrogator configured to interrogate the droplet in order to detect the agent within the aerosol.

2. The detector of claim 1 , wherein the aerosol focuser is configured to pin the droplet proximate an outlet port of the aerosol focuser to enable encapsulation of the aerosol in the droplet.

3. The detector of claim 1 , wherein the liquid feeder is configured to generate the droplet at a substantially same time as the aerosol focuser captures the aerosol.

4. The detector of claim 1 , wherein the assay reagent comprises a fluorescently-tagged antibody, PCR reagent, fluorescent dye, or the like.

5. The detector of claim 1 , wherein the droplet further comprises a microbe.

6. The detector of claim 1 , wherein the aerosol focuser comprises an inlet port, an outlet port, and a focuser lens, wherein the focuser lens is configured to narrow and concentrate the aerosol as the aerosol moves from the inlet port to the outlet port.

7. The detector of claim 1 , wherein the liquid feeder comprises: a liquid suspension channel extending substantially horizontally between a first surface and a second surface of the liquid feeder; an oil channel having a portion extending substantially vertically between the first surface and the second surface of the liquid feeder, wherein the liquid suspension channel and the oil channel intersect at a right angle such that a liquid suspension in the liquid suspension channel and oil in the oil channel converge to generate the droplet.

8. The detector of claim 7 , wherein the liquid feeder further comprises a main channel and a detection channel, wherein the main channel is operably coupled to the liquid suspension channel and extends substantially horizontally between the first surface and the second surface of the liquid feeder downstream of the intersection of the liquid suspension channel and the oil channel, wherein the droplet continues down the main channel towards the detection channel.

9. The detector of claim 8 , wherein the detection channel intersects with the main channel and extends vertically through the first surface of the liquid feeder to form an opening in the first surface of the liquid feeder.

10. The detector of claim 9 , wherein the aerosol focuser is configured to pin the droplet proximate the opening of the liquid feeder.

11. The detector of claim 1 , wherein the detector further includes a microscope such that the reaction of the assay reagent is observable by a user.

12. The detector of claim 1 , wherein the aerosol focuser is located within 0.2-4 mm of a surface of the liquid feeder.

13. The detector of claim 1 , wherein the interrogator comprises a light source configured to emit a light towards the droplet in order to detect the agent in the aerosol.

14. A method for detecting an agent within an aerosol, the method comprising:
generating a droplet comprised of an assay reagent via a liquid feeder;
capturing and focusing the aerosol via an aerosol focuser;
encapsulating the aerosol within the droplet in order to cause the assay reagent to react in the droplet; and
interrogating the droplet with an interrogator in order to detect the agent within the aerosol.

15. The method of claim 14 , wherein the method further comprises: after generating the droplet, pinning the droplet near a surface of the liquid feeder proximate an outlet of the aerosol focuser.

16. The method of claim 15 , wherein the surface is within 0.2-4 mm of the outlet of the aerosol focuser.

17. The method of claim 14 , wherein the generation of the droplet and the capturing and focusing of the aerosol occurring at a substantially same time.

18. The method of claim 14 , wherein the assay reagent comprises one of a fluorescently-tagged antibody, a PCR reagent, a fluorescent dye, or the like.

19. The method of claim 14 , wherein the droplet further comprises a microbe.

20. The method of claim 14 , wherein the focusing of the aerosol comprises successively concentrating the aerosol as the aerosol moves from an inlet port to an outlet port of the aerosol focuser.

Assignees

• Univ Johns Hopkins

Inventors

• Damit Brian E

Classifications

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• G01N1/2208

  with impactors · CPC title

• G01N21/6458 Primary

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N2021/7786

  Fluorescence · CPC title

• G01N2015/0046

  in gas, e.g. smoke · CPC title