Microfluidic circuit allowing drops of several fluids to be brought into contact, and corresponding microfluidic method

Patent metadata

Field	Value
Publication number	US-9943843-B2
Application number	US-201314434342-A
Country	US
Kind code	B2
Filing date	Oct 8, 2013
Priority date	Oct 8, 2012
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

The subject of the present invention is a microfluidic circuit in which are defined microchannels able to contain fluids and including at least one device for forming drops of a solution, guiding the drops to a storage zone in which one of the drops can be brought into contact with a drop of another solution, the walls of the microchannel portion forming the first drop-formation device diverging so as to cause drops of the first solution to detach under the effect of the surface tension of the first solution; the first guide include wall portions of the microchannels that diverge so as to cause the drops to move along under the effect of the surface tension of the first solution.

First claim

The invention claimed is:

1. A microfluidic circuit, in which are defined microchannels containing fluids, said circuit comprising at least:
a first drop forming device configured to form drops of a first solution in a carrier fluid, comprising a first microchannel portion having walls passed through by said first solution; and
a chamber comprising a storage area configured to trap one of said drops and bring said drop into contact with a drop of a second solution and a first guiding means configured to guide said drops formed by the first drop forming device to the storage area of the chamber,
wherein the walls of said first microchannel portion of said first drop forming device diverge so as to detach drops of said first solution under the effect of the surface tension of said first solution, and
said first guiding means comprise a second microchannel portion having walls that diverge so as to displace said drops under the effect of the surface tension of said first solution.

2. The microfluidic circuit according to claim 1 , wherein said first drop forming device comprises a nozzle passed through by said first solution and emerging in the chamber, in which the walls of the chamber are further apart than walls of the nozzle.

3. The microfluidic circuit according to claim 2 , wherein the walls of said chamber define said first guiding means and said storage area.

4. The microfluidic circuit according to claim 1 , wherein an area of one of said microchannels is configured such that a drop may exhibit a lower surface energy than in the neighboring areas.

5. The microfluidic circuit according to claim 4 , wherein said storage area consists of two contiguous trapping areas that may each receive a drop.

6. The microfluidic circuit according to claim 5 , wherein said two contiguous trapping areas are two substantially circular trapping areas that partially intersect, so as to be in the form of an “8”.

7. The microfluidic circuit according to claim 1 , further comprising:
a second drop forming device configured to form drops of the second solution in said carrier fluid, comprising a third microchannel portion having walls passed through by said second solution, and
a second guiding means configured to guide said drops formed by the second drop forming device to the storage area of the chamber, wherein
one of said drops of said second solution may be brought into contact with said drop of the first solution,
the walls of said third microchannel portion of said second drop forming device diverge so as to detach drops of said second solution under the effect of the surface tension of said second solution, and
said second guiding means comprise a fourth microchannel portion having walls that diverge so as to displace the drops of said second solution under the effect of the surface tension of said second solution.

8. The microfluidic circuit according to claim 7 , wherein an area of one of said microchannels is configured such that a drop may exhibit a lower surface energy than in the neighboring areas, said storage area consists of two contiguous trapping areas that may each receive a drop and said first guiding means are configured to guide the drops of said first solution to a first trapping area of said storage area, and said second guiding means are configured to guide the drops of said second solution to a second trapping area of said storage area.

9. The microfluidic circuit according to claim 7 , wherein said first and second drop forming devices are configured to form drops of different sizes.

10. The microfluidic circuit according to claim 9 , wherein an area of one of said microchannels is configured such that a drop may exhibit a lower surface energy than in the neighboring areas, said storage area consists of two contiguous trapping areas that may each receive a drop and said storage area has at least two trapping areas of different sizes, one being of a size suitable for receiving a drop formed by said first drop forming device, and the other being of a size suitable for receiving a drop formed by said second drop forming device.

11. The microfluidic circuit according to claim 8 , further comprising a third drop forming device configured to form drops of a third solution in said carrier fluid, and third guiding means configured to guide said drops formed by the third drop forming device to the storage area of the chamber.

12. The microfluidic circuit according to claim 1 , further comprising means for discharging drops situated in said storage area.

13. A microfluidic method for bringing two drops of different solutions into contact, comprising at least the following steps, performed simultaneously or in succession:
introducing a first solution in microchannels of the microfluidic circuit according to claim 1 ;
detaching a first drop of said first solution in a carrier fluid, caused by the divergence of the walls of said first microchannel portion, coupled with the effects of the surface tension of said first solution;
displacing said first drop, caused by the divergence of the walls of said second microchannel portion, coupled with the effects of the surface tension of said first drop, to the storage area; and
trapping said first drop in said storage area.

14. The microfluidic method according to claim 13 , further comprising the following steps:
introducing a second solution in microchannels of said microfluidic circuit;
detaching a second drop of said second solution in said carrier fluid, caused by the divergence of walls of a third microchannel portion, coupled with the effects of the surface tension of said second solution;
displacing said second drop, caused by the divergence of walls of a fourth microchannel portion, coupled with the effects of the surface tension of said second drop, to said storage area;
trapping said second drop in said storage area; and
bringing into contact said second drop of the second solution with said first drop of the first solution.

15. The method according to claim 14 further comprising merging said second drop of the second solution with said first drop of the first solution as a final step.

Assignees

• Ecole Polytech
• Centre Nat Rech Scient

Inventors

• Baroud Charles
• Abbyad Paul
• Fradet Etienne
• Dangla Rémi

Classifications

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title