Methods for storing red blood cell products

Patent metadata

Field	Value
Publication number	US-9943077-B2
Application number	US-201615195454-A
Country	US
Kind code	B2
Filing date	Jun 28, 2016
Priority date	Oct 23, 2009
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

Methods and systems for processing and conditioning red blood cells are disclosed. The methods and systems may be used to make a readily transfusible red blood cell product with reduced plasma. In general, the plasma content of the supernatant of the red blood cell product is no greater than about 15%. The red blood cell products are prepared using the disclosed methods and systems remain transfusible for up to 42 days.

First claim

The invention claimed is:

1. A method for storing red blood cells comprising:
(a) introducing concentrated red blood cells into a first container comprising container walls having a porosity selected to substantially prevent said red blood cells from passing through said walls of said first container and defining an interior chamber, said first container further comprising a port defining a flow path communicating with said interior chamber of said first container, wherein said red blood cells are introduced into the interior chamber of said first container through said flow path communicating with said interior chamber of said first container;
(b) housing said first container within an interior chamber of a second container, said second container comprising a port defining a flow path communicating with said interior chamber of said second container;
(c) introducing an additive solution into the interior chamber of said second container through said flow path wherein said additive solution is selected to stabilize said red blood cells during a storage period of up to 42 days; and
(d) maintaining said first container with said concentrated red blood cells within said interior chamber of said second container for a selected period of time.

2. The method of claim 1 wherein said concentrated red blood cells have a selected hematocrit, said method comprising substantially maintaining said hematocrit of said concentrated red blood cells during said selected period of time.

3. The method of claim 2 further comprising diluting said concentrated red blood cells with said additive solution prior to introduction into said first container.

4. The method of claim 1 further comprising removing cytokines from said interior chamber of said first container.

5. The method of claim 1 further comprising removing free hemoglobin from said interior chamber of said first container.

6. The method of claim 1 further comprising removing chloride ions from said interior chamber of said first container to the interior chamber of said second container.

7. The method of claim 1 further comprising maintaining said first container with said concentrated red blood cells within said interior chamber of said second container for up to 42 days.

8. The method of claim 1 wherein said additive solution is substantially chloride-free.

9. The method of claim 1 further comprising removing said red blood cells from said interior chamber of said first container through said flow path communicating with said interior chamber of said first container.

Assignees

• Fenwal Inc

Inventors

• Min Kyungyoon
• Radwanski Katherine

Classifications

• A61M2202/0429

  Red blood cells; Erythrocytes · CPC title

• A61M1/30

  Single needle dialysis {; Reciprocating systems, alternately withdrawing blood from and returning it to the patient, e.g. single-lumen-needle dialysis or single needle systems for hemofiltration or pheresis} · CPC title

• A61M1/308

  Volume control, e.g. with open or flexible containers, by counting the number of pump revolutions, weighing · CPC title

• B01D61/28

  Apparatus therefor · CPC title

• A61M1/3693

  using separation based on different densities of components, e.g. centrifuging · CPC title