Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
US-2015064731-A1 · Mar 5, 2015 · US
Substituted imidazo[1,2-a]pyrazines for use in bioluminogenic methods
US9938564B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9938564-B2 |
| Application number | US-201514859715-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 21, 2015 |
| Priority date | Nov 2, 2010 |
| Publication date | Apr 10, 2018 |
| Grant date | Apr 10, 2018 |
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- Title
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Abstract
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The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives including The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
First claim
Opening claim text (preview).
What is claimed: 1. A compound selected from the group consisting of: 2. A kit comprising a compound according to claim 1 . 3. The kit of claim 2 , wherein the kit further comprises a buffer reagent. 4. The kit of claim 2 , wherein the kit further comprises a luciferase. 5. The kit of claim 4 , wherein the luciferase is an Oplophorus or Renilla luciferase. 6. A method for detecting luminescence in a sample, comprising: contacting the sample with a compound; contacting the sample with a coelenterazine-utilizing luciferase, if it is not present in the sample; and detecting luminescence; wherein the compound is selected from the group consisting of: 7. The method of claim 6 , wherein the sample contains live cells. 8. The method of claim 6 , wherein the sample contains a coelenterazine-utilizing luciferase. 9. A method for detecting luminescence in a transgenic animal expressing a coelenterazine-utilizing luciferase, comprising: administering to the transgenic animal a compound; and detecting luminescence; wherein the compound is selected from the group consisting of:
Assignees
Inventors
Classifications
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase · CPC title
- C12Q1/66Primary
involving luciferase · CPC title
Ortho-condensed systems · CPC title
Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase · CPC title
- C12N15/52Primary
Genes encoding for enzymes or proenzymes · CPC title
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- What does patent US9938564B2 cover?
- The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives including The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
- Who is the assignee on this patent?
- Promega Corp
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/66. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 10 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).