Method of producing fatty acids or lipids containing fatty acids using thioesterase variants
US-9222101-B2 · Dec 29, 2015 · US
Modified algae strain and method of triacylglycerol accumulation using said strain
US9938545B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9938545-B2 |
| Application number | US-201414906056-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 21, 2014 |
| Priority date | Jul 19, 2013 |
| Publication date | Apr 10, 2018 |
| Grant date | Apr 10, 2018 |
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- Title
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Abstract
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The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.
First claim
Opening claim text (preview).
The invention claimed is: 1. A genetically engineered strain of Phaeodactylum tricornutum , in which the activity of the comparative gene identification 58 (CGI-58) protein has been modified in order to permit the accumulation of oil in the strain, advantageously accumulation of triacylglycerol, wherein the activity of the CGI-58 is modified by silencing or attenuating the expression of the CGI-58 gene. 2. A genetically engineered strain according to claim 1 , which accumulates or contains at least 1.5-fold the triacylglycerol content of the corresponding wild type strain. 3. A genetically engineered strain according to claim 2 , which accumulates or contains at least 4 fold the triacylglycerol content of the corresponding wild type strain. 4. A method of preparation of a genetically engineered strain of Phaeodactylum tricornutum as described in claim 1 , comprising transforming said strain with a vector expressing RNAi construction designed to target the expression of the CGI-58 gene. 5. The method according to claim 4 , wherein the vector is introduced in the strain by particle bombardment or electroporation. 6. A method for increasing accumulation of triacylglycerol in a strain of Phaeodactylum tricornutum , comprising the step of altering or silencing the expression of the CGI-58 gene in said organism.
Assignees
Inventors
Classifications
- C12P7/6463Primary
obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title
Unicellular algae; Culture media therefor (as new plants A01H13/00) · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
1-Acylglycerol-3-phosphate O-acyltransferase (2.3.1.51) · CPC title
Hydrolases (3) · CPC title
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Frequently asked questions
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- What does patent US9938545B2 cover?
- The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.
- Who is the assignee on this patent?
- Fermentalg, Centre Nat Rech Scient, Commisariat A Lenergie Atomique Et Aux Energies Alternatives, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12P7/6463. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 10 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).