Cell detachment device

Patent metadata

Field	Value
Publication number	US-9938494-B2
Application number	US-201314650053-A
Country	US
Kind code	B2
Filing date	Dec 6, 2013
Priority date	Dec 12, 2012
Publication date	Apr 10, 2018
Grant date	Apr 10, 2018

Abstract

A cell detachment device includes: a base; a vessel holder, to which a culture vessel is to be fitted; a guide mechanism configured to guide reciprocating movement of the vessel holder; a collided member configured to collide with the vessel holder when the vessel holder is moving; an urging member configured to urge the culture vessel toward the collided member; and a power imparting mechanism configured to impart, to the vessel holder, power for causing the vessel holder to move.

First claim

The invention claimed is:

1. A cell detachment device comprising:
a culture vessel with cultured cells attached;
a vessel holder, to which the culture vessel is to be fitted, the vessel holder including a board and a holding frame fixed on the board, the holding frame being formed by one or a plurality of protrusions that rim an outline of the culture vessel place on the board;
a guide mechanism configured to guide reciprocating movement of the vessel holder;
a collided member positioned at one end of a reciprocating movement range of the vessel holder;
a power imparting mechanism configured to impart, to the vessel holder, power for causing the vessel holder to move; and
an urging member configured to urge the vessel holder toward the collided member, wherein:
the vessel holder is reciprocated, while being guided by the guide mechanism, by an urging force of the urging member, such that only a side surface of the board of the vessel holder collides with the collided member at an end of the reciprocating movement range so that the cultured cells are detached from the culture vessel,
the power imparting mechanism includes a controller, and
the controller controls operation of the power imparting mechanism such that the number of collisions between the vessel holder or the culture vessel fitted to the vessel holder and the collided member becomes a predetermined number of impact shocks.

2. The cell detachment device according to claim 1 , wherein
the power imparting mechanism is configured to impart, to the vessel holder, force for causing the vessel holder to move in a direction away from the collided member.

3. The cell detachment device according to claim 1 , wherein
the power imparting mechanism includes:
a motor; and
a cam configured to convert a rotation output of the motor into force acting on the vessel holder.

4. The cell detachment device according to claim 3 , wherein
the cam acts on the vessel holder when a rotation angle of the cam is in a predetermined action range, and the cam does not act on the vessel holder when the rotation angle of the cam is out of the predetermined action range.

5. The cell detachment device according to claim 1 , wherein the urging member is a spring.

6. The cell detachment device according to claim 1 , comprising a second collided member, which is disposed at another end of the reciprocating movement range of the vessel holder.

7. A cell detachment device comprising:
a culture vessel with cultured cells attached;
a vessel holder, to which the culture vessel is to be fitted, the vessel holder including a board and a holding frame fixed on the board, the holding frame being formed by one or a plurality of protrusions that rim an outline of the culture vessel place on the board;
a guide mechanism configured to guide reciprocating movement of the vessel holder;
a collided member positioned at one end of a reciprocating movement range of the vessel holder;
a power imparting mechanism configured to impart, to the vessel holder, power for causing the vessel holder to move; and
an urging member configured to urge the vessel holder toward the collided member, wherein:
the vessel holder is reciprocated, while being guided by the guide mechanism, by an urging force of the urging member, such that only a side surface of the board of the vessel holder collides with the collided member at an end of the reciprocating movement range so that the cultured cells are detached from the culture vessel,
the power imparting mechanism includes:
a motor; and
a cam configured to convert a rotation output of the motor into force acting on the vessel holder,

the cell detachment device further includes:
a rotation number sensor configured to detect the number of rotations of the cam; and
a controller configured to control operation of the motor in accordance with a detection output that the controller receives from the rotation number sensor, and

the controller controls the operation of the motor such that the number of rotations of the cam becomes a predetermined number of impact shocks.

8. The cell detachment device according to claim 7 , wherein
the power imparting mechanism is configured to impart, to the vessel holder, force for causing the vessel holder to move in a direction away from the collided member.

9. The cell detachment device according to claim 7 , wherein
the cam acts on the vessel holder when a rotation angle of the cam is in a predetermined action range, and the cam does not act on the vessel holder when the rotation angle of the cam is out of the predetermined action range.

10. The cell detachment device according to claim 7 , wherein the urging member is a spring.

11. The cell detachment device according to claim 7 , comprising a second collided member, which is disposed at another end of the reciprocating movement range of the vessel holder.

Assignees

• Kawasaki Heavy Ind Ltd

Inventors

• Watakabe Keizo
• Iizaka Junji
• Hasunuma Hitoshi
• Nakashima Katsumi

Classifications

• C12M33/08 Primary

  by vibration · CPC title