Methods of electrospinning and compositions made therefrom

Patent metadata

Field	Value
Publication number	US-9938373-B2
Application number	US-201314652744-A
Country	US
Kind code	B2
Filing date	Dec 20, 2013
Priority date	Dec 21, 2012
Publication date	Apr 10, 2018
Grant date	Apr 10, 2018

Abstract

Disclosed herein are methods of electrospinning poly(glycerol sebacate) (PGS) which allow stable PGS fibers and fibrous PGS constructs, scaffolds and grafts to be formed. In one example, a disclosed method includes generating PGS fibers by blending PGS prepolymer with a heat resistant synthetic carrier polymer, wherein the blend is electrospun into micro- or nano-fibers, and the PGS prepolymer is cross-linked into PGS with heat without using chemical cross-linkers. In another example, a disclosed method includes electrospinning a PGS and gelatin blend, wherein the PGS and gelatin composition are cross-linked by heat curing without using chemical cross-linkers. In another example, the method includes preparing an electrospinning precursor solution comprising blending PGS prepolymer with poly(lactic-co-glycolic acid) (PLGA) and a chemical cross-linker; electrospinning the prepared blend; and exposing the electrospun blend to an organic solvent to remove the PLGA.

First claim

We claim:

1. A method of preparing a fibrous construct, comprising:
electrospinning a poly(glycerol sebacate) (PGS) prepolymer solution comprising PGS prepolymer and a heat-resistant carrier polymer, wherein the PGS prepolymer solution is electrospun into a PGS prepolymer construct, wherein the electrospinning does not include core-shell electrospinning;
cross-linking the electrospun PGS prepolymer construct into a PGS construct by heat curing the electrospun PGS prepolymer and heat-resistant carrier polymer construct without chemical cross-linkers; and
removing the heat-resistant carrier polymer after cross-linking by heat curing the electrospun PGS prepolymer and heat-resistant carrier polymer construct, thereby preparing a fibrous PGS construct.

2. The method of claim 1 , wherein the heat-resistant carrier polymer is polyvinyl alcohol (PVA), polyhydroxybuytrate (PHB), polyethylene terephthalate (PET), polydioxanone (PDO), or poly(lactic acid) (PLA), or a combination thereof.

3. The method of claim 2 , wherein the PGS prepolymer and the heat-resistant carrier polymer solution comprises PGS and the heat resistant carrier polymer at a ratio of about 75:25; 70:30; 65:35; 35:65; 30:70; 25:75; 50:50; 45:55; 55:45; 40:60; or 60:40, respectively.

4. The method of claim 3 , wherein the heat-resistant carrier polymer is PVA.

5. The method of claim 1 , further comprising preparing a PGS prepolymer solution by combining PGS prepolymer and a heat-resistant carrier polymer with hexafluoroisopropanol (HFIP)-water or other appropriate solvents or solvent mixtures prior to electrospinning.

6. The method of claim 1 , wherein heat curing comprises exposing the electrospun PGS prepolymer and heat-resistant carrier polymer construct to between 70° C. and 200° C. temperature for between 2 weeks and 10 hours.

7. A method of preparing a fibrous construct, comprising:
electrospinning poly(glycerol sebacate) (PGS) and gelatin solution, wherein the electrospun PGS and gelatin are cross-linked by heat curing without using chemical cross-linkers, thereby preparing a fibrous construct.

8. A method of preparing a fibrous construct, comprising:
preparing an electrospinning precursor solution comprising blending poly(glycerol sebacate) (PGS) prepolymer with poly(lactic-co-glycolic acid) (PLGA) and a chemical cross-linker; and
electrospinning the blended PGS prepolymer, PLGA and chemical cross-linker to form an electrospun PGS, PLGA, chemically cross-linked construct.

9. The method of claim 2 , wherein the PGS prepolymer and the heat-resistant carrier polymer solution comprises PGS and the heat resistant carrier polymer at a ratio of about 25:75 to 75:25, respectively.

Assignees

• Wang Yadong
• Jeffries Eric M
• Allen Robert A
• Univ Of Pittsburgh—Of The Commonwealth System Of Higher Education

Inventors

• Wang Yadong
• Jeffries Eric M
• Allen Robert A

Classifications

• A61F2250/0067

  Means for introducing or releasing pharmaceutical products into the body · CPC title

• D01D5/0007

  Electro-spinning (non-woven fabrics produced by electro-spinning D04H1/728) · CPC title

• D01F6/62

  from polyesters · CPC title

• A61L27/18

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• D01D5/003

  the material being a polymer solution or dispersion (D01D5/0053 takes precedence) · CPC title