Process for producing a particulate composition comprising crystalline alpha, alpha-trehalose di-hydrate

Patent metadata

Field	Value
Publication number	US-9938311-B2
Application number	US-201715653131-A
Country	US
Kind code	B2
Filing date	Jul 18, 2017
Priority date	Sep 21, 2011
Publication date	Apr 10, 2018
Grant date	Apr 10, 2018

Abstract

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an α-glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing α,α-trehalose; precipitating crystalline α,α-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline α,α-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the α,α-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

First claim

The invention claimed is:

1. A particulate composition comprising crystalline α,α-trehalose dihydrate, in which
(1) said particulate composition has an α,α-trehalose purity of 99.2% by weight or more but not more than 99.7% by weight, on a dry solid basis; and
(2) said particulate composition has a degree of crystallinity for crystalline α,α-trehalose dihydrate of 91.0% or higher but not higher than 96.8%, when calculated based on its powder X-ray diffraction profile.

2. The particulate composition comprising crystalline α,α-trehalose dihydrate of claim 1 , which has a reducing power of the whole particulate composition being not more than 0.5% by weight.

3. The particulate composition comprising crystalline α,α-trehalose dihydrate of claim 1 , wherein said particulate composition contains particles with a particle size of at least 53 μm but smaller than 425 μm in an amount of 80% by weight or more based on the particulate composition as a whole, and particles with a particle size of at least 53 μm but smaller than 300 μm in an amount of 60% by weight or more base on the particulate composition as a whole.

Assignees

• Hayashibara Co

Inventors

• Shibuya Takashi
• Izawa Seisuke

Classifications

• C07H1/00

  Processes for the preparation of sugar derivatives · CPC title

• C12Y204/01019

  Cyclomaltodextrin glucanotransferase (2.4.1.19) · CPC title

• C12P19/12 Primary

  Disaccharides · CPC title

• C12P19/18

  produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title

• C13K13/007

  Separation of sugars provided for in subclass C13K (C13K3/00 takes precedence) · CPC title