Processing of biological growth media based on measured manufacturing characteristics

Patent metadata

Field	Value
Publication number	US-9933446-B2
Application number	US-92018809-A
Country	US
Kind code	B2
Filing date	Feb 27, 2009
Priority date	Mar 4, 2008
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

This disclosure is directed to information management techniques that may be used during automated processing of biological growth media. In one embodiment, a method comprises reading an identification element associated with a biological growth medium, identifying manufacturing information associated with the biological growth medium based on the identification element, wherein the manufacturing information includes one or more manufacturing variables measured from the biological growth medium when the biological growth medium was manufactured, adjusting one or more processing parameters associated with processing of the biological growth medium based on the manufacturing variables measured from the biological growth medium when the biological growth medium was manufactured, and processing the biological growth medium in an automated system based on the processing parameters.

First claim

The invention claimed is:

1. A method comprising:
reading, by an identification element reader of a biological growth medium processing system, an identification element associated with a biological growth plate for growing and detecting bacterial colonies, wherein the biological growth plate is obtained from a particular manufacturing lot;
identifying, by a computer of the system, manufacturing information associated with the particular manufacturing lot using the identification element;
wherein the manufacturing information includes one or more spectroscopy measurements measured from the biological growth plate when the biological growth plate was manufactured;

identifying, by the computer, based on the spectroscopy measurements, variations from expected colors;
inoculating the biological growth plate;
generating, by a biological reader of the system, an image of the biological growth plate after bacterial colonies have formed on the biological growth plate;
modifying, by the computer, the image by applying color offsets to colors of the image of the biological growth plate, thereby accounting for the variations from the expected colors; and
counting, by the computer, using the modified image, the bacterial colonies on the biological growth plate.

2. The method of claim 1 , wherein the one or more spectroscopy measurements comprise spectroscopy absorption measurements and spectroscopy transmission measurements.

3. The method of claim 1 , wherein the manufacturing information includes:
first information indicative of a plate type associated with the biological growth plate;
second information indicative of a manufacturer of the biological growth plate; and
third information that includes the one or more manufacturing variables measured from the biological growth plate when the biological growth plate was manufactured.

4. The method of claim 3 , wherein the image is a first image, the method further comprising:
selecting, by the computer, an image processing profile to be used by the biological reader for the biological growth plate using the first information;
verifying, by the computer, the manufacturer of the biological growth plate using the second information;
adjusting, by the computer, parameters of the system using the third information;
generating, by the biological reader, one or more images of the biological growth plate using the biological reader, the one or more images including the first image; and
processing, by the computer, the one or more images using the selected imaging processing profile.

5. The method of claim 3 , wherein the manufacturing information includes: fourth information indicative of a manufacturing date associated with the biological growth plate.

6. The method of claim 5 , further comprising:
determining, by the computer, an expiration date associated with the biological growth plate using the manufacturing date; and
identifying, by the computer, the biological growth plate as expired if the expiration date has passed.

7. The method of claim 1 , further comprising:
incubating, by an incubator of the system, the biological growth plate;
recording, by the computer, times associated with inoculating, incubating and generating the image; and
associating, by the computer, the times with the identification element.

8. The method of claim 1 , wherein the biological growth plate comprises a hydrogel.

9. The method of claim 1 , further comprising adjusting, by the computer, based on the manufacturing information, a parameter used to generate the image of the biological growth plate.

10. The method of claim 9 , wherein the parameter is selected from the group consisting of an illumination source, an illumination angle, and a camera exposure.

11. The method of claim 1 , further comprising adjusting, by the computer, based on the manufacturing information, an algorithm used to count the bacterial colonies.

12. The method of claim 1 , further comprising:
after identifying the manufacturing information, providing, by the system, a dwell time for gelling the biological growth plate, wherein the manufacturing information is used to direct the dwell time.

13. A method comprising:
reading, by an identification element reader of a biological growth medium processing system, an identification element associated with a biological growth plate on which bacterial colonies have formed, wherein the biological growth plate is obtained from a particular manufacturing lot;
identifying, by a computer of the system, manufacturing information associated with the particular manufacturing lot using the identification element;
wherein the manufacturing information includes one or more spectroscopy measurements measured from the biological growth plate when the biological growth plate was manufactured;

identifying, by the computer, based on the spectroscopy measurements, variations from expected colors;
generating, by a biological reader of the system, an image of the biological growth plate;
modifying, by the computer, the image by applying color offsets to colors of the image of the biological growth plate, thereby accounting for the variations from the expected colors; and
counting, by the computer, using the modified image, the bacterial colonies on the biological growth plate.

Assignees

• Bolea Phillip A
• Hughes Michael E
• 3M Innovative Properties Co

Inventors

• Bolea Phillip A
• Hughes Michael E

Classifications

• G01N2035/00851

  process control parameters · CPC title

• G01N2035/00752

  bar codes · CPC title

• G01N35/00732 Primary

  Identification of carriers, materials or components in automatic analysers · CPC title